Description:
Figure 1. Double label of beta-galactosidase (βGal)
activity and NeuN (pan Neuronal) immunostaining. A majority of the xGal-positive cells of the grey matter appear to be neuronal, as they co-label with NeuN. However, some xGal positive staining is not restricted to neurons under high magnification (right panel). (scale bar = 400μm and 50μm respectively)
Figure 2. Beta-galactosidase staining throughout the brain of an adult germline mouse. The section was counterstained with NeutralRed. This figure shows examples of xGal
staining in the cortex (A and B), hippocampus (C and D), and the cerebellum (E and
F). In each case the staining is mosaic, with radial columns of cells in the cortex and the hippocampus. In the cerebellum, most xGal positive cells are distributed in the granular and molecular layers, few Pukinje cells are positive. Scale bar = 400μm (A, C, E), 100μm (B, D, F).
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