The Pleiades Promoter Project

All MiniPromoters

Ple57-EGFP (Negative)

Other constructs for this MiniPromoter: NONE

Other constructs for this Gene: Ple56-EGFP (Negative), Ple58-EGFP (Negative), Ple58-lacZ (Negative), Ple59-EGFP (Negative), Ple59-lacZ (Negative)

7.6 weeks, N2, Male
(entire series)

Ple57-EGFP Description:

The Ple57 promoter construct is derived from the promoter region of the Dopa Decarboxylase (DDC) gene. The Pleiades Promoter Project constructed a 3125 bp MiniPromoter from two candidate regulatory regions of the DDC gene along with a subsection of the DDC promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1307 (EGFP reporter flanked by full frt sites) to make plasmid pEMS1126 with Ple57 driving EGFP. The Ple57-EGFP (pEMS1126) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS2488, mEMS2489, mEMS2490, and mEMS2712) were PCR validated. Chimeras were generated using mEMS2488.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple57-EGFP Expression Pattern:

No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).

Ple57 MiniPromoter Resources:

Ple57-EGFP
	plasmid pEMS1126 (in backbone pEMS1307)
	ESCs mEMS2488, mEMS2489, mEMS2490, mEMS2712
	mice generated using ESC mEMS2488
Resource Files
	Ple57 pEMS1126 Sequence	Jones lab
	Ple57 pEMS1126 MiniPromoter Design	Wasserman lab
	Ple57 pEMS1126 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple57 pEMS1126 Vector Map	Holt lab
	Ple57 pEMS1126 Genotyping Assay	Simpson lab
	Ple57 pEMS1126 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID

Aguanno A, Lee MR, Marden CM, Rattray M, Gault A, Albert VR. Analysis of the neuronal promoter of the rat aromatic L-amino acid decarboxylase gene. J Neurochem. 1995 Nov;65(5):1944-54.	7595477
Sumi-Ichinose C, Hasegawa S, Ichinose H, Sawada H, Kobayashi K, Sakai M, Fujii T, Nomura H, Nomura T, Nagatsu I, et al. Analysis of the alternative promoters that regulate tissue-specific expression of human aromatic L-amino acid decarboxylase. J Neurochem. 1995 Feb;64(2):514-24.	7830043
Albert VR, Lee MR, Bolden AH, Wurzburger RJ, Aguanno A. Distinct promoters direct neuronal and nonneuronal expression of rat aromatic L-amino acid decarboxylase. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12053-7.	1465439
Li XM, Juorio AV, Boulton AA. NSD-1015 alters the gene expression of aromatic L-amino acid decarboxylase in rat PC12 pheochromocytoma cells. Neurochem Res. 1993 Aug;18(8):915-9.	8371833
Jahng JW, Wessel TC, Houpt TA, Son JH, Joh TH. Alternate promoters in the rat aromatic L-amino acid decarboxylase gene for neuronal and nonneuronal expression: an in situ hybridization study. J Neurochem. 1996 Jan;66(1):14-9.	8522946
Aguanno A, Afar R, Albert VR. Tissue-specific expression of the nonneuronal promoter of the aromatic L-amino acid decarboxylase gene is regulated by hepatocyte nuclear factor 1. J Biol Chem. 1996 Feb 23;271(8):4528-38.	8626808
Sumi-Ichinose C, Hasegawa S, Ohtsuki M, Nomura H, Nomura T, Hagino Y, Fujita K, Nagatsu T. Analysis of an alternative promoter that regulates tissue-specific expression of the human aromatic L-amino acid decarboxylase gene in cultured cell lines. J Neural Transm. 1996;103(1-2):1-15.	9026364
Chireux M, Raynal JF, Le Van Thai A, Cadas H, Bernard C, Martinou I, Martinou JC, Weber MJ. Multiple promoters of human choline acetyltransferase and aromatic L-amino acid decarboxylase genes. J Physiol Paris. 1994;88(4):215-27.	7874082
Le Van Thai A, Coste E, Allen JM, Palmiter RD, Weber MJ. Identification of a neuron-specific promoter of human aromatic L-amino acid decarboxylase gene. Brain Res Mol Brain Res. 1993 Mar;17(3-4):227-38.	8510497
Li XM, Juorio AV, Paterson IA, Walz W, Zhu MY, Boulton AA. Gene expression of aromatic L-amino acid decarboxylase in cultured rat glial cells. J Neurochem. 1992 Sep;59(3):1172-5.	1494905
Tison F, Normand E, Jaber M, Aubert I, Bloch B. Aromatic L-amino-acid decarboxylase (DOPA decarboxylase) gene expression in dopaminergic and serotoninergic cells of the rat brainstem. Neurosci Lett. 1991 Jun 24;127(2):203-6.	1679228
Menheniott TR, Woodfine K, Schulz R, Wood AJ, Monk D, Giraud AS, Baldwin HS, Moore GE, Oakey RJ. Genomic imprinting of Dopa decarboxylase in heart and reciprocal allelic expression with neighboring Grb10. Mol Cell Biol. 2008 Jan;28(1):386-96. Epub 2007 Oct 29.	17967881
Davis MM, Yang P, Chen L, O'Keefe SL, Hodgetts RB. The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster. Genome. 2007 Nov;50(11):1049-1060.	18059550
Fujimori KE. Characterization of the Regulatory Region of the Dopa Decarboxylase Gene in Medaka: An in vivo Green Fluorescent Protein Reporter Assay Combined with a Simple TA-Cloning Method. Mol Biotechnol. 2008 Nov 6; [Epub ahead of print]	18987999
Urdinguio RG, Lopez-Serra L, Lopez-Nieva P, Alaminos M, Diaz-Uriarte R, Fernandez AF, Esteller M. Mecp2-null mice provide new neuronal targets for Rett syndrome. PLoS ONE. 2008;3(11):e3669. Epub 2008 Nov 7.	18989361
Brown ST, Kelly KF, Daniel JM, Nurse CA. Hypoxia inducible factor (HIF)-2alpha is required for the development of the catecholaminergic phenotype of sympathoadrenal cells. J Neurochem. 2009 May 8; [Epub ahead of print]	19457096