The Pleiades Promoter Project

All MiniPromoters

Ple151-EGFP (Positive)*

Other constructs for this MiniPromoter: NONE

Other constructs for this Gene: Ple148-EGFP (Negative), Ple150-EGFP (Negative)


9.1 wk., N2, Male (entire series)	Adult, N2, Male (entire series)

Ple151-EGFP Description:

The Ple151 promoter construct is derived from the promoter region of the oligodendrocyte transcription factor 1 (OLIG1) gene. The Pleiades Promoter Project created a 3033 bp MiniPromoter derived from one upstream and two downstream putative regulatory subregions along with a section of the promoter, and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1172 with Ple151 driving EGFP. The Ple151-EGFP (pEMS1172) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204.03A. Four ESC lines (mEMS1608, mEMS1609, mEMS1614, and mEMS1615) were PCR validated. Chimeras were generated using mEMS1615.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple151-EGFP Expression Pattern:

Positive EGFP immunoreactivity was detected across numerous brain regions. EGFP-positive cells show darker staining in the nucleus with a lighter staining halo of processes. In the brainstem, cortex and thalamus, numerous immunopositive profiles are detected while fewer profiles are seen in the olfactory bulb, striatum and cerebellum. In contrast to the predicted expression of Olig1, the EGFP expression does not appear to be enriched in white matter tracts such as the corpus callosum.

Ple151 MiniPromoter Resources:

Ple151-EGFP
	plasmid pEMS1172 (in backbone pEMS1306)
	ESCs mEMS1608, 1609, 1614, 1615
	mice generated using ESC mESM1615
	The mouse strain is available through the Jackson Laboratory: http://jaxmice.jax.org/strain/008708.html
Resource Files
	Ple151 pEMS1172 Sequence	Jones lab
	Ple151 pEMS1172 MiniPromoter Design	Wasserman lab
	Ple151 pEMS1172 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple151 pEMS1172 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple151 pEMS1172 Vector Map	Holt lab
	Ple151 pEMS1172 Vector Map	Holt lab
	Ple151 pEMS1172 Genotyping Assay	Simpson lab
	Ple151 pEMS1172 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab
	Ple151 pEMS1172 ESC description	Simpson lab

Publications:

Reference	PubMed ID
Lu QR, Yuk D, Alberta JA, Zhu Z, Pawlitzky I, Chan J, McMahon AP, Stiles CD, Rowitch DH. Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron. 2000 Feb;25(2):317-29.	10719888
Qi Y, Cai J, Wu Y, Wu R, Lee J, Fu H, Rao M, Sussel L, Rubenstein J, Qiu M. Control of oligodendrocyte differentiation by the Nkx2.2 homeodomain transcription factor. Development. 2001 Jul;128(14):2723-33.	11526078
Gong X, Lin T, Sun Z, Fu M, Zuo H, Xie Z. Olig1 is downregulated in oligodendrocyte progenitor cell differentiation. Neuroreport. 2008 Aug 6;19(12):1203-1207.	18628665
Ikushima H, Komuro A, Isogaya K, Shinozaki M, Hellman U, Miyazawa K, Miyazono K. An Id-like molecule, HHM, is a synexpression group-restricted regulator of TGF-beta signalling. EMBO J. 2008 Oct 16; [Epub ahead of print]	18923419

* The Ple151 MiniPromoter (Patent Pending) is proprietary - any commercial use of the MiniPromoter will require a direct license from The University of British Columbia. Please direct all licensing inquiries to Elizabeth M. Simpson, PhD, simpson@cmmt.ubc.ca Office: 604-875-3830