The Pleiades Promoter Project

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All MiniPromoters

Ple55-EGFP (Positive)

Other constructs for this MiniPromoter: Ple55-lacZ (Positive)

Other constructs for this Gene: Ple53-EGFP (Positive), Ple53-lacZ (Positive), Ple54-EGFP (Positive)

8 weeks, F0, Female
(entire series)

Ple55-EGFP Description:

TThe Ple55 promoter construct is derived from the promoter region of the Doublecortin (DCX) gene. The Pleiades Promoter Project created a 3877 bp MiniPromoter derived from one downstream putative regulatory subregion along with a section of the promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1201 with Ple55 driving EGFP. The Ple55-EGFP (pEMS1201) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1218. Four ESC lines (mEMS1935, mEMS1933, mEMS1943, and mEMS1941) were PCR validated. Chimeras were generated using mEMS1933.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple55 MiniPromoter Resources:

Ple55-EGFP
	plasmid pEMS1201 (in backbone pEMS1306)
	ESCs mEMS1935, mEMS1933, mEMS1943, mEMS1941
	chimeras generated using ESC mEMS1933
Resource Files
	Ple55 pEMS1201 Sequence	Jones lab
	Ple55 pEMS1201 MiniPromoter Design	Wasserman lab
	Ple55 pEMS1201 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple55 pEMS1201 Vector Map	Holt lab
	Ple55 pEMS1201 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID
Karl C, Couillard-Despres S, Prang P, Munding M, Kilb W, Brigadski T, Plotz S, Mages W, Luhmann H, Winkler J, Bogdahn U, Aigner L. Neuronal precursor-specific activity of a human doublecortin regulatory sequence. J Neurochem. 2005 Jan;92(2):264-82.	15663475
Couillard-Despres S, Winner B, Karl C, Lindemann G, Schmid P, Aigner R, Laemke J, Bogdahn U, Winkler J, Bischofberger J, Aigner L. Targeted transgene expression in neuronal precursors: watching young neurons in the old brain. Eur J Neurosci. 2006 Sep;24(6):1535-45.	17004917
Wang X, Mao X, Xie L, Greenberg DA, Jin K. Involvement of Notch1 signaling in neurogenesis in the subventricular zone of normal and ischemic rat brain in vivo. J Cereb Blood Flow Metab. 2009 Jun 17; [Epub ahead of print]	19536070
Piens M, Muller M, Bodson M, Baudouin G, Plumier JC. A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons. BMC Neurosci. 2010 May 28;11(1):64 [Epub ahead of print]	20509865

Ple55-EGFP (Positive)

Ple55-EGFP Description:

Ple55-EGFP Expression Pattern:

Ple55 MiniPromoter Resources:

Publications: