Ple13-EGFP (Negative)
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Other constructs for this MiniPromoter: NONE |
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Other constructs for this Gene: Ple12-EGFP (Negative), Ple12-lacZ (Positive) |
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Ple13-EGFP Description:
The Ple12 promoter construct is derived from the promoter region of the arginine vasopressin (AVP)gene. The Pleiades Promoter Project constructed a 3967 bp MiniPromoter from two downstream candidate regulatory regions and a subsection of the AVP promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1307 (EGFP reporter flanked by full frt sites) to make plasmid pEMS1242 with Ple13 driving EGFP. The Ple13-EGFP (pEMS1242) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS2327, mEMS2335, mEMS2336, and mEMS2337) were PCR validated. Chimeras were generated using mEMS2327.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple13-EGFP Expression Pattern:
No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).
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Ple13 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Ueta Y, Fujihara H, Serino R, Dayanithi G, Ozawa H, Matsuda K, Kawata M, Yamada J, Ueno S, Fukuda A, Murphy D. Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat. Endocrinology. 2005 Jan;146(1):406-13. Epub 2004 Sep 16. | 15375027 | | Shapiro RA, Xu C, Dorsa DM. Differential transcriptional regulation of rat vasopressin gene expression by estrogen receptor alpha and beta. Endocrinology. 2000 Nov;141(11):4056-64. | 11089536 | | Yoshida M, Iwasaki Y, Asai M, Takayasu S, Taguchi T, Itoi K, Hashimoto K, Oiso Y. Identification of a functional AP1 element in the rat vasopressin gene promoter. Endocrinology. 2006 Jun;147(6):2850-63. Epub 2006 Mar 16. | 16543367 | | Murgatroyd C, Wigger A, Frank E, Singewald N, Bunck M, Holsboer F, Landgraf R, Spengler D. Impaired repression at a vasopressin promoter polymorphism underlies overexpression of vasopressin in a rat model of trait anxiety. J Neurosci. 2004 Sep 1;24(35):7762-70. | 15342744 | | Coulson JM, Fiskerstrand CE, Woll PJ, Quinn JP. E-box motifs within the human vasopressin gene promoter contribute to a major enhancer in small-cell lung cancer. Biochem J. 1999 Dec 15;344 Pt 3:961-70. | 10585887 | | Pak TR, Chung WC, Hinds LR, Handa RJ. Estrogen receptor-beta mediates dihydrotestosterone-induced stimulation of the arginine vasopressin promoter in neuronal cells. Endocrinology. 2007 Jul;148(7):3371-82. Epub 2007 Apr 5. | 17412808 | | Coulson JM, Fiskerstrand CE, Woll PJ, Quinn JP. Arginine vasopressin promoter regulation is mediated by a neuron-restrictive silencer element in small cell lung cancer. Cancer Res. 1999 Oct 15;59(20):5123-7. | 10537286 | | Fujio T, Fujihara H, Shibata M, Yamada S, Onaka T, Tanaka K, Morita H, Dayanithi G, Kawata M, Murphy D, Ueta Y. Exaggerated response of arginine vasopressin-enhanced green fluorescent protein fusion gene to salt loading without disturbance of body fluid homeostasis in rats. J Neuroendocrinol. 2006 Oct;18(10):776-85. | 16965296 | | Coulson JM, Edgson JL, Marshall-Jones ZV, Mulgrew R, Quinn JP, Woll PJ. Upstream stimulatory factor activates the vasopressin promoter via multiple motifs, including a non-canonical E-box. Biochem J. 2003 Feb 1;369(Pt 3):549-61. | 12403649 | | Yokoi H, Nagasaki H, Tachikawa K, Arima H, Murase T, Miura Y, Hirabayashi M, Oiso Y. Adaptation to sustained high plasma vasopressin in water and electrolyte homeostasis in the rat transgenic for the metallothionein-vasopressin fusion gene. J Endocrinol. 2002 Apr;173(1):23-33. | 11927381 | | Grace CO, Fink G, Quinn JP. Characterization of potential regulatory elements within the rat arginine vasopressin proximal promoter. Neuropeptides. 1999 Feb;33(1):81-90. | 10657475 | | Coulson JM, Fiskerstrand CE, Woll PJ, Quinn JP. Arginine vasopressin promoter regulation is mediated by a neuron-restrictive silencer element in small cell lung cancer. Cancer Res. 1999 Oct 15;59(20):5123-7. | 10537286 | | Coulson JM, Stanley J, Woll PJ. Tumour-specific arginine vasopressin promoter activation in small-cell lung cancer. Br J Cancer. 1999 Aug;80(12):1935-44. | 10471042 | | Sausville E, Carney D, Battey J. The human vasopressin gene is linked to the oxytocin gene and is selectively expressed in a cultured lung cancer cell line. J Biol Chem. 1985 Aug 25;260(18):10236-41. | 2991279 | | Habener JF, Cwikel BJ, Hermann H, Hammer RE, Palmiter RD, Brinster RL. Metallothionein-vasopressin fusion gene expression in transgenic mice. Nephrogenic diabetes insipidus and brain transcripts localized to magnocellular neurons. J Biol Chem. 1989 Nov 5;264(31):18844-52. | 2808395 | | Transgenesis and neuroendocrine physiology: a transgenic rat model expressing growth hormone in vasopressin neurones. Comment in: J Physiol. 2003 Aug 15;551(Pt 1):3. J Physiol. 2003 Aug 15;551(Pt 1):323-36. Epub 2003 Jun 17. | 12813157 | | Ghorbel MT, Coulson JM, Murphy D. Cross-talk between hypoxic and circadian pathways: cooperative roles for hypoxia-inducible factor 1alpha and CLOCK in transcriptional activation of the vasopressin gene. Mol Cell Neurosci. 2003 Mar;22(3):396-404. | 12691740 | | Davies J, Waller S, Zeng Q, Wells S, Murphy D. Further delineation of the sequences required for the expression and physiological regulation of the vasopressin gene in transgenic rat hypothalamic magnocellular neurones. J Neuroendocrinol. 2003 Jan;15(1):42-50. | 12535168 | | Coulson JM, Edgson JL, Marshall-Jones ZV, Mulgrew R, Quinn JP, Woll PJ. Upstream stimulatory factor activates the vasopressin promoter via multiple motifs, including a non-canonical E-box. Biochem J. 2003 Feb 1;369(Pt 3):549-61. | 12403649 | | Schoenhard JA, Eren M, Johnson CH, Vaughan DE. Alternative splicing yields novel BMAL2 variants: tissue distribution and functional characterization. Am J Physiol Cell Physiol. 2002 Jul;283(1):C103-14. | 12055078 | | Iwasaki Y, Oiso Y, Saito H, Majzoub JA. Positive and negative regulation of the rat vasopressin gene promoter. Endocrinology. 1997 Dec;138(12):5266-74. | 9389510 | | Burke ZD, Ho MY, Morgan H, Smith M, Murphy D, Carter D. Repression of vasopressin gene expression by glucocorticoids in transgenic mice: evidence of a direct mechanism mediated by proximal 5' flanking sequence. Neuroscience. 1997 Jun;78(4):1177-85. | 9174083 | | Grant FD, Reventos J, Kawabata S, Miller M, Gordon JW, Majzoub JA. Transgenic mouse models of vasopressin expression. Hypertension. 1993 Oct;22(4):640-5. | 8406671 | | Grant FD, Reventos J, Gordon JW, Kawabata S, Miller M, Majzoub JA. Expression of the rat arginine vasopressin gene in transgenic mice. Mol Endocrinol. 1993 May;7(5):659-67. | 8100353 | | Zeng Q, Carter DA, Murphy D. Cell specific expression of a vasopressin transgene in rats. J Neuroendocrinol. 1994 Oct;6(5):469-77. | 7827617 | | Zeng Q, Foo NC, Funkhouser JM, Carter DA, Murphy D. Expression of a rat vasopressin transgene in rat testes. J Reprod Fertil. 1994 Nov;102(2):471-81. | 7532224 | | Foo NC, Funkhouser JM, Carter DA, Murphy D. A testis-specific promoter in the rat vasopressin gene. J Biol Chem. 1994 Jan 7;269(1):659-67. | 7506260 | | Ivell R, Richter D. Structure and comparison of the oxytocin and vasopressin genes from rat. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2006-10. | 6326097 | | Mohr E, Richter D. Sequence analysis of the promoter region of the rat vasopressin gene. FEBS Lett. 1990 Jan 29;260(2):305-8. | 2153590 | | Ang HL, Carter DA, Murphy D. Neuron-specific expression and physiological regulation of bovine vasopressin transgenes in mice. EMBO J. 1993 Jun;12(6):2397-409. | 7685275 | | Jin X, Shearman LP, Weaver DR, Zylka MJ, de Vries GJ, Reppert SM. A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock. Cell. 1999 Jan 8;96(1):57-68. | 9989497 | | Bunck M, Czibere L, Horvath C, Graf C, Frank E, Kessler MS, Murgatroyd C, Muller-Myhsok B, Gonik M, Weber P, Putz B, Muigg P, Panhuysen M, Singewald N, Bettecken T, Deussing JM, Holsboer F, Spengler D, Landgraf R. A hypomorphic vasopressin allele prevents anxiety-related behavior. PLoS ONE. 2009;4(4):e5129. Epub 2009 Apr 9. | 19357765 | | Auger CJ, Coss D, Auger AP, Forbes-Lorman RM. Epigenetic control of vasopressin expression is maintained by steroid hormones in the adult male rat brain. Proc Natl Acad Sci U S A. 2011 Feb 22; [Epub ahead of print] | 21368111 |
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