The Pleiades Promoter Project

All MiniPromoters

Ple21-EGFP (Negative)

Other constructs for this MiniPromoter: Ple21-lacZ (Negative)

Other constructs for this Gene: Ple20-EGFP (Negative)

The images are coming soon!

The description is coming soon!

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple21-EGFP
	plasmid pEMS1246 (in backbone pEMS1307)
	ESCs mEMS3451, mEMS3452, mEMS3461, mEMS3472
	mice generated using ESC mEMS3472
Resource Files
	Ple21 pEMS1246 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple21 pEMS1246 Vector Map	Holt lab
	Ple21 pEMS1246 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Reference	PubMed ID
Dominguez G, Lakatos A, Kuhar MJ. Characterization of the cocaine- and amphetamine-regulated transcript (CART) peptide gene promoter and its activation by a cyclic AMP-dependent signaling pathway in GH3 cells. J Neurochem. 2002 Mar;80(5):885-93.	11948252
CART gene promoter transcription is regulated by a cyclic adenosine monophosphate response element. Erratum in: Obes Res. 2003 Jan;11(1):following table of contents. Obes Res. 2002 Dec;10(12):1291-8.	12490674
Lakatos A, Dominguez G, Kuhar MJ. CART promoter CRE site binds phosphorylated CREB. Brain Res Mol Brain Res. 2002 Jul 15;104(1):81-5.	12117553
Jones DC, Kuhar MJ. Cocaine-amphetamine-regulated transcript expression in the rat nucleus accumbens is regulated by adenylyl cyclase and the cyclic adenosine 5'-monophosphate/protein kinase a second messenger system. J Pharmacol Exp Ther. 2006 Apr;317(1):454-61. Epub 2005 Dec 1.	16322355
Li Y, Liu Q, Yang Y, Lv Y, Chen L, Bai C, Nan X, Wang Y, Pei X. Regulatory role of neuron-restrictive silencing factor in the specific expression of cocaine- and amphetamine-regulated transcript (CART) gene. J Neurochem. 2008 May 15; [Epub ahead of print]	18485095
Rogge GA, Jones DC, Green T, Nestler E, Kuhar MJ. Regulation of CART peptide expression by CREB in the rat nucleus accumbens in vivo. Brain Res. 2008 Nov 14; [Epub ahead of print]	19046951