Negative Control (pEMS1302)

Promoterless Description:

The Pleiades expression vector pEMS1302 (containing EGFPCre reporter flanked by minimal frt sites) with no MiniPromoter construct was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS21TG2A. Four ESC lines (mEMS1503, mEMS1504, mEMS1516, and mEMS1527) were PCR validated. Chimeras were generated using mEMS669. This is a negative control.

Note: CAG positive controls (pEMS1157, pEMS1277 & pEMS1488) have also been generated.

Promoterless Resources:

Promoterless EGFP/cre reporter flanked by minimal frt sites
	plasmid pEMS1302
	ESCs mEMS669, mEMS673, mEMS680, mEMS682
	mice generated using ESC mEMS669
Resource Files
	Promoterless pEMS1302 Vector NTI File	Holt lab
	Promoterless pEMS1302 Vector Map	Holt lab
	Promoterless pEMS1302 Genotyping Assay	Simpson lab
	Promoterless ESC description	Simpson lab
	Promoterless Mouse description	Simpson lab