Negative Control (pEMS1306)

Promoterless Description:

To act as a negative control the promoterless Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1204.  The resulting PCR validated four ESC lines, mEMS1503, mEMS1504, mEMS1516, and mEMS1527.  Chimeras were generated using mEMS1503, mEMS1504, and mEMS1516 and the cell line mEMS1504 was used to generate the mouse strain B6.129P2-Hprt1^{tm1(mEMS1504)Ems}.  The images above are derived from this mouse strain and show no expression as expected of this negative control.

In a parallel experiment, also to generate a negative control, the promoterless Pleiades expression vector pEMS1307 (EGFP reporter flanked by full frt sites) was also produced.

Note: CAG positive controls (pEMS1157, pEMS1277 & pEMS1488) have also been generated.

Promoterless Resources:

Promoterless EGFP reporter flanked by minimal frt sites
	plasmid pEMS1306
	ESCs mEMS1503, mEMS1504, mEMS1516, and mEMS1527
	mice generated using ESC mEMS1504
Resource Files
	Promoterless pEMS1306 Vector NTI File	Holt lab
	Promoterless pEMS1306 Vector Map	Holt lab
	Promoterless pEMS1306 Genotyping Assay	Simpson lab
	Promoterless ESC description	Simpson lab
	Promoterless Mouse description	Simpson lab