Negative Control (pEMS1307)

Promoterless Description:

To act as a negative control the promoterless Pleiades expression vector pEMS1307 (EGFP reporter flanked by full frt sites) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1218. The resulting PCR validated four ESC lines, mEMS2076, mEMS2081, mEMS2087, and mEMS2088. Chimeras were generated using mEMS2076. No mouse strains have yet been derived or images produced for this negative control.

In a parallel experiment, also to generate a negative control, the promoterless Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) was also produced.

Note: two CAG positive controls (pEMS1157 & pEMS1277) have also been generated.

Promoterless Resources:

Promoterless EGFP reporter flanked by minimal frt sites
	plasmid pEMS1307
	ESCs mEMS2076, mEMS2081, mEMS2087, and mEMS2088
	chimeras generated using mEMS2076
Resource Files
	Promoterless pEMS1307 Vector NTI File	Holt lab
	Promoterless pEMS1307 Vector Map	Holt lab
	Promoterless pEMS1307 Genotyping Assay	Simpson lab