Negative Control (pEMS1313)

Promoterless Description:

The Pleiades expression vector pEMS1313 (containing a lacZ reporter flanked by full frt sites) with no MiniPromoter construct was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1202.02. Four ESC lines (mEMS3689, mEMS3684, mEMS3768, and mEMS3770) were PCR validated. Chimeras were generated using mEMS3689. This is a negative control.

Note: CAG positive controls (pEMS1157, pEMS1277 & pEMS1488) have also been generated.

Promoterless Resources: