Ple49-EGFP (Negative)
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Other constructs for this MiniPromoter: Ple49-lacZ (Positive) |
Other constructs for this Gene: Ple48-EGFP (Negative), Ple50-EGFP (Negative), Ple51-EGFP (Negative),
Ple48-lacZ (Positive) |
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Ple49-EGFP Description:
The Ple49 promoter construct is derived from the promoter region of the dopamine beta hydroxylase (DBH) gene. The Pleiades Promoter Project developed a 756 bp MiniPromoter derived from an upstream candidate regulatory region along with segment of the promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1360 with Ple49 driving EGFP. The Ple49-EGFP (pEMS1360) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS1769, mEMS1770, mEMS1772, and mEMS1783) were PCR validated. Chimeras were generated using mEMS1769.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple49-EGFP Expression Pattern:
MiniPromoter Ple49 was assayed for EGFP from pEMS1306 backbone. No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).
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Ple49 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Mercer EH, Hoyle GW, Kapur RP, Brinster RL, Palmiter RD. The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice. Neuron. 1991 Nov;7(5):703-16. | 1742021 | | Moriguchi T, Takako N, Hamada M, Maeda A, Fujioka Y, Kuroha T, Huber RE, Hasegawa SL, Rao A, Yamamoto M, Takahashi S, Lim KC, Engel JD. Gata3 participates in a complex transcriptional feedback network to regulate sympathoadrenal differentiation. Development. 2006 Oct;133(19):3871-81. Epub 2006 Aug 30. | 16943277 | | Kobayashi K, Kurosawa Y, Fujita K, Nagatsu T. Human dopamine beta-hydroxylase gene: two mRNA types having different 3'-terminal regions are produced through alternative polyadenylation. Nucleic Acids Res. 1989 Feb 11;17(3):1089-102. | 2922261 | | Ishiguro H, Yamada K, Ichino N, Nagatsu T. Identification and characterization of a novel phorbol ester-responsive DNA sequence in the 5'-flanking region of the human dopamine beta-hydroxylase gene. J Biol Chem. 1998 Aug 21;273(34):21941-9. | 9705334 | | Swanson DJ, Zellmer E, Lewis EJ. The homeodomain protein Arix interacts synergistically with cyclic AMP to regulate expression of neurotransmitter biosynthetic genes. J Biol Chem. 1997 Oct 24;272(43):27382-92. | 9341190 | | Shaskus J, Greco D, Asnani LP, Lewis EJ. A bifunctional genetic regulatory element of the rat dopamine beta-hydroxylase gene influences cell type specificity and second messenger-mediated transcription. J Biol Chem. 1992 Sep 15;267(26):18821-30. | 1382062 | | Ishiguro H, Kim KT, Joh TH, Kim KS. Neuron-specific expression of the human dopamine beta-hydroxylase gene requires both the cAMP-response element and a silencer region. J Biol Chem. 1993 Aug 25;268(24):17987-94. | 7688735 | | Ishiguro H, Kim KS, Joh TH. Identification of a negative regulatory element in the 5'-flanking region of the human dopamine beta-hydroxylase gene. Brain Res Mol Brain Res. 1995 Dec 28;34(2):251-61. | 8750828 | | Hwang DY, Carlezon WA Jr, Isacson O, Kim KS. A high-efficiency synthetic promoter that drives transgene expression selectively in noradrenergic neurons. Hum Gene Ther. 2001 Sep 20;12(14):1731-40. | 11560767 | | Kim HS, Seo H, Yang C, Brunet JF, Kim KS. Noradrenergic-specific transcription of the dopamine beta-hydroxylase gene requires synergy of multiple cis-acting elements including at least two Phox2a-binding sites. J Neurosci. 1998 Oct 15;18(20):8247-60. | 9763470 | | Yang C, Kim HS, Seo H, Kim CH, Brunet JF, Kim KS. Paired-like homeodomain proteins, Phox2a and Phox2b, are responsible for noradrenergic cell-specific transcription of the dopamine beta-hydroxylase gene. J Neurochem. 1998 Nov;71(5):1813-26. | 9798905 | | Seo H, Yang C, Kim HS, Kim KS. Multiple protein factors interact with the cis-regulatory elements of the proximal promoter in a cell-specific manner and regulate transcription of the dopamine beta-hydroxylase gene. J Neurosci. 1996 Jul 1;16(13):4102-12. | 8753872 | | Kim JS, Nam JS, Chae HD, Kim KT. A protein kinase C-activating phorbol ester enhances transcription of the human DBH gene through a cyclic AMP response element in SK-N-BE(2)C cells. Brain Res Mol Brain Res. 1997 Nov;51(1-2):154-60. | 9427517 | | Xu H, Firulli AB, Zhang X, Howard MJ. HAND2 synergistically enhances transcription of dopamine-beta-hydroxylase in the presence of Phox2a. Dev Biol. 2003 Oct 1;262(1):183-93. | 14512028 | | Rychlik JL, Gerbasi V, Lewis EJ. The interaction between dHAND and Arix at the dopamine beta-hydroxylase promoter region is independent of direct dHAND binding to DNA. J Biol Chem. 2003 Dec 5;278(49):49652-60. Epub 2003 Sep 23. | 14506227 | | Adachi M, Browne D, Lewis EJ. Paired-like homeodomain proteins Phox2a/Arix and Phox2b/NBPhox have similar genetic organization and independently regulate dopamine beta-hydroxylase gene transcription. DNA Cell Biol. 2000 Sep;19(9):539-54. | 11034547 | | Adachi M, Lewis EJ. The paired-like homeodomain protein, Arix, mediates protein kinase A-stimulated dopamine beta-hydroxylase gene transcription through its phosphorylation status. J Biol Chem. 2002 Jun 21;277(25):22915-24. Epub 2002 Apr 9. | 11943777 | | Swanson DJ, Adachi M, Lewis EJ. The homeodomain protein Arix promotes protein kinase A-dependent activation of the dopamine beta-hydroxylase promoter through multiple elements and interaction with the coactivator cAMP-response element-binding protein-binding protein. J Biol Chem. 2000 Jan 28;275(4):2911-23. | 10644760 | | Hoyle GW, Mercer EH, Palmiter RD, Brinster RL. Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements. J Neurosci. 1994 May;14(5 Pt 1):2455-63. | 8182421 | | Cheng SY, Serova LI, Glazkova D, Sabban EL. Regulation of rat dopamine beta-hydroxylase gene transcription by early growth response gene 1 (Egr1). Brain Res. 2008 Feb 8;1193:1-11. Epub 2007 Dec 5. | 18190898 | | Ruohonen ST, Abe K, Kero M, Toukola L, Ruohonen S, Roytta M, Koulu M, Pesonen U, Zukowska Z, Savontaus E. Sympathetic nervous system-targeted neuropeptide Y overexpression in mice enhances neointimal formation in response to vascular injury. Peptides. 2008 Dec 24; [Epub ahead of print] | 19135490 | | Hess C, Reif A, Strobel A, Boreatti-Hummer A, Heine M, Lesch KP, Jacob CP. A functional dopamine-beta-hydroxylase gene promoter polymorphism is associated with impulsive personality styles, but not with affective disorders. J Neural Transm. 2009 Feb;116(2):121-30. Epub 2008 Nov 4. | 18982239 | | Ross OA, Heckman MG, Soto AI, Diehl NN, Haugarvoll K, Vilarino-Guell C, Aasly JO, Sando S, Gibson JM, Lynch T, Krygowska-Wajs A, Opala G, Barcikowska M, Czyzewski K, Uitti RJ, Wszolek ZK, Farrer MJ. Dopamine beta-hydroxylase -1021C>T association and Parkinson's disease. Parkinsonism Relat Disord. 2008 Nov;14(7):544-7. Epub 2008 Aug 22. | 18722802 | | Gilsbach R, Roeser C, Beetz N, Brede M, Hadamek K, Haubold M, Leemhuis J, Philipp M, Schneider J, Urbanski M, Szabo B, Weinshenker D, Hein L. Genetic dissection of {alpha}2-adrenoceptor functions in adrenergic versus non-adrenergic cells. Mol Pharmacol. 2009 Feb 27; [Epub ahead of print] | 19251826 | | | 0 | | Jirout ML, Friese RS, Mahapatra NR, Mahata M, Taupenot L, Mahata SK, Kren V, Zidek V, Fischer J, Maatz H, Ziegler MG, Pravenec M, Hubner N, Aitman TJ, Schork NJ, O'Connor DT. Genetic regulation of catecholamine synthesis, storage and secretion in the spontaneously hypertensive rat. Hum Mol Genet. 2010 Apr 8; [Epub ahead of print] | 20378607 | | Jirout ML, Friese RS, Mahapatra NR, Mahata M, Taupenot L, Mahata SK, Kren V, Zidek V, Fischer J, Maatz H, Ziegler MG, Pravenec M, Hubner N, Aitman TJ, Schork NJ, O'Connor DT. Genetic regulation of catecholamine synthesis, storage and secretion in the spontaneously hypertensive rat. Hum Mol Genet. 2010 Apr 8; [Epub ahead of print] | 20378607 | | Serova LI, Nostramo R, Veerasirikul M, Cappell DB, Sabban EL. Varied Mechanisms of Oestradiol Mediated Regulation of Dopamine beta-Hydroxylase Transcription. J Neuroendocrinol. 2010 Nov 9; [Epub ahead of print] | 21062376 |
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