The Pleiades Promoter Project

All MiniPromoters

Ple88 Promoter (pEMS1375)

Other constructs for this promoter: NONE


P2, F0, Male (entire series)	P3, N1, Female (entire series)	P3, N1, Female (entire series)	(entire series)

Ple88 Description:

The Ple88 promoter construct is derived from the promoter region of the Glial Fibrillary Acidic Protein gene (GFAP). The Pleiades Promoter Project isolated a 2210 bp subregion of the GFAP promoter immediately upstream of the first exon of the gene and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1375 with Ple88 driving EGFP. The Ple88-EGFP (pEMS1375) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1204. The resulting PCR validated four ESC lines, mEMS1653, mEMS1661, mEMS1671, and mEMS1672. Chimeras were generated using mEMS1653 and this cell line was used to generate the images above.

Note: a promoterless negative control (pEMS1306) has been generated, as well as a two CAG positive controls (pEMS1157 & pEMS1277).

Ple88 MiniPromoter Resources:

GFAP-A EGFP
	plasmid pEMS1375 (in backbone pEMS1306)
	ESCs mEMS1653, mEMS1661, mEMS1671, and mEMS1672
	chimeras generated using mEMS1653
	images from mEMS1653
Resource Files
	Ple88 pEMS1375 Sequence	Jones lab
	Ple88 pEMS1375 MiniPromoter Design	Wasserman lab
	Ple88 pEMS1375 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple88 pEMS1375 Vector Map	Holt lab
	Ple88 pEMS1375 Genotyping Assay	Simpson lab
	Ple88 pEMS1375 ESC description	Simpson lab
	Ple88 pEMS1375 Mouse description	Simpson lab

Publications:

Reference	PubMed ID
McKie EA, Graham DI, Brown SM. Selective astrocytic transgene expression in vitro and in vivo from the GFAP promoter in a HSV RL1 null mutant vector--potential glioblastoma targeting. Gene Ther. 1998 Apr;5(4):440-50.	9614567
Barton MD, Dunlop JW, Psaltis G, Kulik J, DeGennaro L, Kwak SP. Modified GFAP promoter auto-regulates tet-activator expression for increased transactivation and reduced tTA-associated toxicity. Brain Res Mol Brain Res. 2002 May 30;101(1-2):71-81.	12007834
Kuzmanovic M, Dudley VJ, Sarthy VP. GFAP promoter drives Muller cell-specific expression in transgenic mice. Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3606-13.	12882814
Verderber L, Johnson W, Mucke L, Sarthy V. Differential regulation of a glial fibrillary acidic protein-LacZ transgene in retinal astrocytes and Muller cells. Invest Ophthalmol Vis Sci. 1995 May;36(6):1137-43.	7730023
Hatch N, Sarid J. Glial fibrillary acidic protein transcriptional regulation is independent of a TFIID-binding downstream initiator sequence. J Neurochem. 1994 Dec;63(6):2003-9.	7964717
Nakatani Y, Horikoshi M, Brenner M, Yamamoto T, Besnard F, Roeder RG, Freese E. A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID. Nature. 1990 Nov 1;348(6296):86-8.	2234067
Brenner M, Kisseberth WC, Su Y, Besnard F, Messing A. GFAP promoter directs astrocyte-specific expression in transgenic mice. J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.	8120611
Besnard F, Brenner M, Nakatani Y, Chao R, Purohit HJ, Freese E. Multiple interacting sites regulate astrocyte-specific transcription of the human gene for glial fibrillary acidic protein. J Biol Chem. 1991 Oct 5;266(28):18877-83.	1918004
Gomes FC, Paulin D, Moura Neto V. Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation. Braz J Med Biol Res. 1999 May;32(5):619-31.	10412574
Nakatani Y, Brenner M, Freese E. An RNA polymerase II promoter containing sequences upstream and downstream from the RNA startpoint that direct initiation of transcription from the same site. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4289-93.	2349237
Sarid J. Identification of a cis-acting positive regulatory element of the glial fibrillary acidic protein gene. J Neurosci Res. 1991 Feb;28(2):217-28.	2033651
Miura M, Tamura T, Mikoshiba K. Cell-specific expression of the mouse glial fibrillary acidic protein gene: identification of the cis- and trans-acting promoter elements for astrocyte-specific expression. J Neurochem. 1990 Oct;55(4):1180-8.	2398353
Masood K, Besnard F, Su Y, Brenner M. Analysis of a segment of the human glial fibrillary acidic protein gene that directs astrocyte-specific transcription. J Neurochem. 1993 Jul;61(1):160-6.	8515262
Galou M, Pournin S, Ensergueix D, Ridet JL, Tchelingerian JL, Lossouarn L, Privat A, Babinet C, Dupouey P. Normal and pathological expression of GFAP promoter elements in transgenic mice. Glia. 1994 Dec;12(4):281-93.	7890332
Sarkar S, Cowan NJ. Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein. J Neurochem. 1991 Aug;57(2):675-84.	2072110
Pettigrew DB, Li YQ, Kuntz C 4th, Crutcher KA. Global expression of NGF promotes sympathetic axonal growth in CNS white matter but does not alter its parallel orientation. Exp Neurol. 2007 Jan;203(1):95-109. Epub 2006 Sep 20.	16989811
Johnson WB, Ruppe MD, Rockenstein EM, Price J, Sarthy VP, Verderber LC, Mucke L. Indicator expression directed by regulatory sequences of the glial fibrillary acidic protein (GFAP) gene: in vivo comparison of distinct GFAP-lacZ transgenes. Glia. 1995 Mar;13(3):174-84.	7782103
Hasegawa A, Naruse M, Hitoshi S, Iwasaki Y, Takebayashi H, Ikenaka K. Regulation of glial development by cystatin C. J Neurochem. 2007 Jan;100(1):12-22. Epub 2006 Oct 25.	17064358
Steiner J, Bernstein HG, Bielau H, Berndt A, Brisch R, Mawrin C, Keilhoff G, Bogerts B. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007 Jan 2;8:2.	17199889
Ohno M, Kohyama J, Namihira M, Sanosaka T, Takahashi JA, Hashimoto N, Nakashima K. Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3. Cytokine. 2006 Oct;36(1-2):17-22. Epub 2006 Dec 11.	17161614
Wiencken-Barger AE, Djukic B, Casper KB, McCarthy KD. A role for Connexin43 during neurodevelopment. Glia. 2007 May;55(7):675-86.	17311295
Casper KB, McCarthy KD. GFAP-positive progenitor cells produce neurons and oligodendrocytes throughout the CNS. Mol Cell Neurosci. 2006 Apr;31(4):676-84. Epub 2006 Feb 3.	16458536
Lee Y, Messing A, Su M, Brenner M. GFAP promoter elements required for region-specific and astrocyte-specific expression. Glia. 2008 Apr;56(5):481-93.	18240313
Chow LM, Zhang J, Baker SJ. Inducible Cre recombinase activity in mouse mature astrocytes and adult neural precursor cells. Transgenic Res. 2008 May 16; [Epub ahead of print]	18483774
Romao LF, Sousa VD, Neto VM, Gomes FC. Glutamate activates GFAP gene promoter from cultured astrocytes through TGF-beta1 pathways. J Neurochem. 2008 May 14; [Epub ahead of print]	18419760
Cebolla B, Fernandez-Perez A, Perea G, Araque A, Vallejo M. DREAM mediates cAMP-dependent, Ca2+-induced stimulation of GFAP gene expression and regulates cortical astrogliogenesis. J Neurosci. 2008 Jun 25;28(26):6703-13.	18579744
Bonni A, Sun Y, Nadal-Vicens M, Bhatt A, Frank DA, Rozovsky I, Stahl N, Yancopoulos GD, Greenberg ME. Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway. Science. 1997 Oct 17;278(5337):477-83.	9334309
Cebolla B, Vallejo M. Nuclear factor-I regulates glial fibrillary acidic protein gene expression in astrocytes differentiated from cortical precursor cells. J Neurochem. 2006 May;97(4):1057-70. Epub 2006 Apr 5.	16606365
Krohn K, Rozovsky I, Wals P, Teter B, Anderson CP, Finch CE. Glial fibrillary acidic protein transcription responses to transforming growth factor-beta1 and interleukin-1beta are mediated by a nuclear factor-1-like site in the near-upstream promoter. J Neurochem. 1999 Apr;72(4):1353-61.	10098836