Ple90-EGFP (Positive)
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Other constructs for this MiniPromoter: NONE |
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Other constructs for this Gene: Ple88-EGFP (Positive), Ple88-lacZ (Positive) |
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Ple90-EGFP Description:
The Ple90 promoter construct is derived from the promoter region of the Glial Fibrillary
Acidic Protein gene (GFAP). The Pleiades Promoter Project created a 1433 bp MiniPromoter
derived from a putative upstream regulatory region and a portion of the promoter
of the gene and introduced it into the multiple cloning site (MCS) of Pleiades
expression
vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1377
with Ple90 driving EGFP. The Ple90-EGFP (pEMS1377) was introduced by homologous
recombination as a single copy insertion into the Hprt1b-m3 locus in the X Chromosome
of mouse embryonic stem cells (ESCs) mEMS1204. The resulting PCR validated four
ESC lines, mEMS1889, mEMS1899, mEMS1900, and mEMS1903. Chimeras were generated using
mEMS1899 and this cell line was used to generate the
images above.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple90-EGFP Expression Pattern:
The expression pattern of the Ple90 MiniPromoter is quite similar to the expression of Ple88, with broad expression in glial cellular profiles throughout the brain and spinal cord. Numerous astroglial profiles are seen in the cortical grey and white matter and in the cerebellum the full extent of Bergmann glial processes are highlighted with anti-EGFP immunocytochemistry. EGFP-immunoreativity is most prominent in the cell bodies across all regions, with a subpopulation of cells showing a halo of process staining.
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Ple90 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| McKie EA, Graham DI, Brown SM. Selective astrocytic transgene expression in vitro and in vivo from the GFAP promoter in a HSV RL1 null mutant vector--potential glioblastoma targeting. Gene Ther. 1998 Apr;5(4):440-50. | 9614567 | | Barton MD, Dunlop JW, Psaltis G, Kulik J, DeGennaro L, Kwak SP. Modified GFAP promoter auto-regulates tet-activator expression for increased transactivation and reduced tTA-associated toxicity. Brain Res Mol Brain Res. 2002 May 30;101(1-2):71-81. | 12007834 | | Kuzmanovic M, Dudley VJ, Sarthy VP. GFAP promoter drives Muller cell-specific expression in transgenic mice. Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3606-13. | 12882814 | | Verderber L, Johnson W, Mucke L, Sarthy V. Differential regulation of a glial fibrillary acidic protein-LacZ transgene in retinal astrocytes and Muller cells. Invest Ophthalmol Vis Sci. 1995 May;36(6):1137-43. | 7730023 | | Hatch N, Sarid J. Glial fibrillary acidic protein transcriptional regulation is independent of a TFIID-binding downstream initiator sequence. J Neurochem. 1994 Dec;63(6):2003-9. | 7964717 | | Nakatani Y, Horikoshi M, Brenner M, Yamamoto T, Besnard F, Roeder RG, Freese E. A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID. Nature. 1990 Nov 1;348(6296):86-8. | 2234067 | | Brenner M, Kisseberth WC, Su Y, Besnard F, Messing A. GFAP promoter directs astrocyte-specific expression in transgenic mice. J Neurosci. 1994 Mar;14(3 Pt 1):1030-7. | 8120611 | | Besnard F, Brenner M, Nakatani Y, Chao R, Purohit HJ, Freese E. Multiple interacting sites regulate astrocyte-specific transcription of the human gene for glial fibrillary acidic protein. J Biol Chem. 1991 Oct 5;266(28):18877-83. | 1918004 | | Gomes FC, Paulin D, Moura Neto V. Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation. Braz J Med Biol Res. 1999 May;32(5):619-31. | 10412574 | | Nakatani Y, Brenner M, Freese E. An RNA polymerase II promoter containing sequences upstream and downstream from the RNA startpoint that direct initiation of transcription from the same site. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4289-93. | 2349237 | | Sarid J. Identification of a cis-acting positive regulatory element of the glial fibrillary acidic protein gene. J Neurosci Res. 1991 Feb;28(2):217-28. | 2033651 | | Miura M, Tamura T, Mikoshiba K. Cell-specific expression of the mouse glial fibrillary acidic protein gene: identification of the cis- and trans-acting promoter elements for astrocyte-specific expression. J Neurochem. 1990 Oct;55(4):1180-8. | 2398353 | | Masood K, Besnard F, Su Y, Brenner M. Analysis of a segment of the human glial fibrillary acidic protein gene that directs astrocyte-specific transcription. J Neurochem. 1993 Jul;61(1):160-6. | 8515262 | | Galou M, Pournin S, Ensergueix D, Ridet JL, Tchelingerian JL, Lossouarn L, Privat A, Babinet C, Dupouey P. Normal and pathological expression of GFAP promoter elements in transgenic mice. Glia. 1994 Dec;12(4):281-93. | 7890332 | | Sarkar S, Cowan NJ. Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein. J Neurochem. 1991 Aug;57(2):675-84. | 2072110 | | Pettigrew DB, Li YQ, Kuntz C 4th, Crutcher KA. Global expression of NGF promotes sympathetic axonal growth in CNS white matter but does not alter its parallel orientation. Exp Neurol. 2007 Jan;203(1):95-109. Epub 2006 Sep 20. | 16989811 | | Johnson WB, Ruppe MD, Rockenstein EM, Price J, Sarthy VP, Verderber LC, Mucke L. Indicator expression directed by regulatory sequences of the glial fibrillary acidic protein (GFAP) gene: in vivo comparison of distinct GFAP-lacZ transgenes. Glia. 1995 Mar;13(3):174-84. | 7782103 | | Hasegawa A, Naruse M, Hitoshi S, Iwasaki Y, Takebayashi H, Ikenaka K. Regulation of glial development by cystatin C. J Neurochem. 2007 Jan;100(1):12-22. Epub 2006 Oct 25. | 17064358 | | Steiner J, Bernstein HG, Bielau H, Berndt A, Brisch R, Mawrin C, Keilhoff G, Bogerts B. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007 Jan 2;8:2. | 17199889 | | Ohno M, Kohyama J, Namihira M, Sanosaka T, Takahashi JA, Hashimoto N, Nakashima K. Neuropoietin induces neuroepithelial cells to differentiate into astrocytes via activation of STAT3. Cytokine. 2006 Oct;36(1-2):17-22. Epub 2006 Dec 11. | 17161614 | | Wiencken-Barger AE, Djukic B, Casper KB, McCarthy KD. A role for Connexin43 during neurodevelopment. Glia. 2007 May;55(7):675-86. | 17311295 | | Casper KB, McCarthy KD. GFAP-positive progenitor cells produce neurons and oligodendrocytes throughout the CNS. Mol Cell Neurosci. 2006 Apr;31(4):676-84. Epub 2006 Feb 3. | 16458536 | | Lee Y, Messing A, Su M, Brenner M. GFAP promoter elements required for region-specific and astrocyte-specific expression. Glia. 2008 Apr;56(5):481-93. | 18240313 | | Chow LM, Zhang J, Baker SJ. Inducible Cre recombinase activity in mouse mature astrocytes and adult neural precursor cells. Transgenic Res. 2008 May 16; [Epub ahead of print] | 18483774 | | Romao LF, Sousa VD, Neto VM, Gomes FC. Glutamate activates GFAP gene promoter from cultured astrocytes through TGF-beta1 pathways. J Neurochem. 2008 May 14; [Epub ahead of print] | 18419760 | | Cebolla B, Fernandez-Perez A, Perea G, Araque A, Vallejo M. DREAM mediates cAMP-dependent, Ca2+-induced stimulation of GFAP gene expression and regulates cortical astrogliogenesis. J Neurosci. 2008 Jun 25;28(26):6703-13. | 18579744 | | Bonni A, Sun Y, Nadal-Vicens M, Bhatt A, Frank DA, Rozovsky I, Stahl N, Yancopoulos GD, Greenberg ME. Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway. Science. 1997 Oct 17;278(5337):477-83. | 9334309 | | Cebolla B, Vallejo M. Nuclear factor-I regulates glial fibrillary acidic protein gene expression in astrocytes differentiated from cortical precursor cells. J Neurochem. 2006 May;97(4):1057-70. Epub 2006 Apr 5. | 16606365 | | Krohn K, Rozovsky I, Wals P, Teter B, Anderson CP, Finch CE. Glial fibrillary acidic protein transcription responses to transforming growth factor-beta1 and interleukin-1beta are mediated by a nuclear factor-1-like site in the near-upstream promoter. J Neurochem. 1999 Apr;72(4):1353-61. | 10098836 | | Lastres-Becker I, Fernandez-Perez A, Cebolla B, Vallejo M. Pituitary adenylate cyclase-activating polypeptide stimulates glial fibrillary acidic protein gene expression in cortical precursor cells by activating Ras and Rap1. Mol Cell Neurosci. 2008 Jul 29; [Epub ahead of print] | 18707003 | | Cho W, Hagemann TL, Johnson DA, Johnson JA, Messing A. Dual transgenic reporter mice as a tool for monitoring expression of GFAP. J Neurochem. 2009 May 5; [Epub ahead of print] | 19457099 | | Asano H, Aonuma M, Sanosaka T, Kohyama J, Namihira M, Nakashima K. Astrocyte Differentiation Of Neural Precursor Cells Is Enhanced By Retinoic Acid Through A Change In Epigenetic Modification. Stem Cells. 2009 Jul 16; [Epub ahead of print] | 19609931 | | Restrepo A, Smith CA, Agnihotri S, Shekarforoush M, Kongkham PN, Seol HJ, Northcott P, Rutka JT. Epigenetic regulation of glial fibrillary acidic protein by DNA methylation in human malignant gliomas. Neuro Oncol. 2010 Nov 12; [Epub ahead of print] | 21075782 | | Weber T, Baier V, Pauly R, Sahay A, Baur M, Herrmann E, Ciccolini F, Hen R, Kronenberg G, Bartsch D. Inducible gene expression in GFAP+ progenitor cells of the SGZ and the dorsal wall of the SVZ-A novel tool to manipulate and trace adult neurogenesis. Glia. 2011 Feb 3; [Epub ahead of print] | 21294160 | | Liu Y, Zhu H, Liu M, Du J, Qian Y, Wang Y, Ding F, Gu X. Downregulation of Pax3 expression correlates with acquired GFAP expression during NSC differentiation towards astrocytes. FEBS Lett. 2011 Feb 28; [Epub ahead of print] | 21371476 |
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