The Pleiades Promoter Project

All MiniPromoters

Ple185 Promoter (pEMS1384)*

Other constructs for this promoter: pEMS1104


8.3 wk., 50% Chimera, Male (entire series)	, 95% Chimera (entire series)	, 14% Chimera (entire series)

Ple185 Description:

The Ple185 promoter construct is derived from the promoter region of the S100 protein, beta polypeptide (S100β). The Pleiades Promoter Project isolated a 2440 bp subregion of the S100β promoter along with a 1340 bp basal promoter element and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1384 with Ple185 driving EGFP. The Ple185-EGFP (pEMS1384) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1204. The resulting PCR validated four ESC lines, mEMS1603, mEMS1604, mEMS1610, and mEMS1627. Chimeras were generated using mEMS1627 and this cell line was used to generate the images above.

Note: a promoterless negative control (pEMS1306) has been generated, as well as a two CAG positive controls (pEMS1157 & pEMS1277).

Ple185 MiniPromoter Resources:

S100β-C EGFP
	plasmid pEMS1384 (in backbone pEMS1306)
	ESCs mEMS1603, mEMS1604, mEMS1610, and mEMS1627
	chimeras generated using mEMS1627
	images from mEMS1627
Resource Files
	Ple185 pEMS1384 Sequence	Jones lab
	Ple185 pEMS1384 MiniPromoter Design	Wasserman lab
	Ple185 pEMS1384 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple185 pEMS1384 Vector Map	Holt lab
	Ple185 pEMS1384 Genotyping Assay	Simpson lab
	Ple185 pEMS1384 ESC description	Simpson lab
	Ple185 pEMS1384 Mouse description	Simpson lab

Publications:

Reference	PubMed ID
Castets F, Griffin WS, Marks A, Van Eldik LJ. Transcriptional regulation of the human S100 beta gene. Brain Res Mol Brain Res. 1997 Jun;46(1-2):208-16.	9191095
Lin J, Yang Q, Yan Z, Markowitz J, Wilder PT, Carrier F, Weber DJ. Inhibiting S100B restores p53 levels in primary malignant melanoma cancer cells. J Biol Chem. 2004 Aug 6;279(32):34071-7. Epub 2004 Jun 3.	15178678
Allore RJ, Friend WC, O'Hanlon D, Neilson KM, Baumal R, Dunn RJ, Marks A. Cloning and expression of the human S100 beta gene. J Biol Chem. 1990 Sep 15;265(26):15537-43.	2394738
Jiang H, Shah S, Hilt DC. Organization, sequence, and expression of the murine S100 beta gene. Transcriptional regulation by cell type-specific cis-acting regulatory elements. J Biol Chem. 1993 Sep 25;268(27):20502-11.	8376406
Hagiwara N, Sueoka N. Regulation of the rat S100 beta gene expression: the role of the 2 kb 5'-upstream sequence in glial specific expression. J Neurosci Res. 1995 Dec;42(5):603-12.	8600292
Steiner J, Bernstein HG, Bielau H, Berndt A, Brisch R, Mawrin C, Keilhoff G, Bogerts B. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007 Jan 2;8:2.	17199889
Malup TK, Kobzev VF, Zhdanova LG, Slobodyanyuk SY, Sviridov SM. Methylation of CpG dinucleotides in the promoter region of the gene encoding the S100b protein in BALB/cLac mice. Dokl Biochem Biophys. 2007 Jan-Feb;412:1-3.	17506341
Vives V, Alonso G, Solal AC, Joubert D, Legraverend C. Visualization of S100B-positive neurons and glia in the central nervous system of EGFP transgenic mice. J Comp Neurol. 2003 Mar 17;457(4):404-19.	12561079
Yokoo H, Tanaka Y, Nobusawa S, Nakazato Y, Ohgaki H. Immunohistochemical and ultrastructural characterization of brain tumors in S100beta-v-erbB transgenic rats. Neuropathology. 2008 May 21; [Epub ahead of print]	18498286
Itakura E, Odaira K, Yokoyama K, Osuna M, Hara T, Inoue K. Generation of transgenic rats expressing green fluorescent protein in S-100beta-producing pituitary folliculo-stellate cells and brain astrocytes. Endocrinology. 2007 Apr;148(4):1518-23. Epub 2007 Jan 18.	17234709
Maeda T, Usui H, Araki K, Kuwano R, Takahashi Y, Suzuki Y. Structure and expression of rat S-100 beta subunit gene. Brain Res Mol Brain Res. 1991 Jun;10(3):193-202.	1653388
Pena LA, Brecher CW, Marshak DR. beta-Amyloid regulates gene expression of glial trophic substance S100 beta in C6 glioma and primary astrocyte cultures. Brain Res Mol Brain Res. 1995 Dec 1;34(1):118-26.	8750867
Hinkle DA, Harney JP, Cai A, Hilt DC, Yarowsky PJ, Wise PM. Basic fibroblast growth factor-2 and interleukin-1 beta regulate S100 beta expression in cultured astrocytes. Neuroscience. 1998 Jan;82(1):33-41.	9483501
Ohgaki H, Kita D, Favereaux A, Huang H, Homma T, Dessen P, Weiss WA, Kleihues P, Heppner FL. Brain tumors in S100beta-v-erbB transgenic rats. J Neuropathol Exp Neurol. 2006 Dec;65(12):1111-7.	17146284
Weiss WA, Burns MJ, Hackett C, Aldape K, Hill JR, Kuriyama H, Kuriyama N, Milshteyn N, Roberts T, Wendland MF, DePinho R, Israel MA. Genetic determinants of malignancy in a mouse model for oligodendroglioma. Cancer Res. 2003 Apr 1;63(7):1589-95.	12670909
Darlot F, Artuso A, Lautredou-Audouy N, Casellas D. Topology of Schwann Cells and Sympathetic Innervation along Preglomerular vessels. A Confocal Microscopic Study in Protein S100B / EGFP Transgenic Mice. Am J Physiol Renal Physiol. 2008 Aug 13; [Epub ahead of print]	18701627

* The Ple185 MiniPromoter (Patent Pending) is proprietary - any commercial use of the MiniPromoter will require a direct license from The University of British Columbia. Please direct all licensing inquiries to Dr. Joshua Eades at the University-Industry Liaison Office - 604-827-4430.