Ple185-EGFP (Positive)*
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Other constructs for this MiniPromoter: NONE |
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Other constructs for this Gene: Ple183-EGFP (Positive), Ple184-EGFP (Negative), Ple186-EGFP (Positive) |
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Ple185-EGFP Description:
The Ple185 promoter construct is derived from the promoter region of the S100 protein,
beta polypeptide (S100β). The Pleiades Promoter Project isolated a 2440 bp
subregion of the S100β promoter along with a 1340 bp basal promoter element
and introduced them into the multiple cloning site (MCS) of Pleiades expression
vector pEMS1306 (EGFP reporter flanked by minimal
frt sites) to make plasmid pEMS1384 with Ple185 driving EGFP. The Ple185-EGFP (pEMS1384)
was introduced by homologous recombination as single copy insertion into the Hprt1b-m3
locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1204. The resulting
PCR validated four ESC lines, mEMS1603, mEMS1604, mEMS1610, and mEMS1627. Chimeras
were generated using mEMS1627 and this cell line was used to generate the images
above.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple185-EGFP Expression Pattern:
The pattern of expression of the Ple185-EGFP MiniPromoter is broad throughout the brain in glial populations, most notably in cerebellar Bergman glia, as well as fiber tracts throughout the brain. Descending white matter tracts coursing through the corpus callosum, striatum, and into the brainstem are also prominently labeled. The EGFP-immunoreactivity shows significant overlap with S100ß expression in many regions, including the cerebellum, cortex, and spinal cord. There is also extensive colabeling of EGFP with the myelin marker RIP in white matter tracts throughout the Ple185-EGFP MiniPromoter brain.
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Ple185 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Castets F, Griffin WS, Marks A, Van Eldik LJ. Transcriptional regulation of the human S100 beta gene. Brain Res Mol Brain Res. 1997 Jun;46(1-2):208-16. | 9191095 | | Lin J, Yang Q, Yan Z, Markowitz J, Wilder PT, Carrier F, Weber DJ. Inhibiting S100B restores p53 levels in primary malignant melanoma cancer cells. J Biol Chem. 2004 Aug 6;279(32):34071-7. Epub 2004 Jun 3. | 15178678 | | Allore RJ, Friend WC, O'Hanlon D, Neilson KM, Baumal R, Dunn RJ, Marks A. Cloning and expression of the human S100 beta gene. J Biol Chem. 1990 Sep 15;265(26):15537-43. | 2394738 | | Jiang H, Shah S, Hilt DC. Organization, sequence, and expression of the murine S100 beta gene. Transcriptional regulation by cell type-specific cis-acting regulatory elements. J Biol Chem. 1993 Sep 25;268(27):20502-11. | 8376406 | | Hagiwara N, Sueoka N. Regulation of the rat S100 beta gene expression: the role of the 2 kb 5'-upstream sequence in glial specific expression. J Neurosci Res. 1995 Dec;42(5):603-12. | 8600292 | | Steiner J, Bernstein HG, Bielau H, Berndt A, Brisch R, Mawrin C, Keilhoff G, Bogerts B. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007 Jan 2;8:2. | 17199889 | | Malup TK, Kobzev VF, Zhdanova LG, Slobodyanyuk SY, Sviridov SM. Methylation of CpG dinucleotides in the promoter region of the gene encoding the S100b protein in BALB/cLac mice. Dokl Biochem Biophys. 2007 Jan-Feb;412:1-3. | 17506341 | | Vives V, Alonso G, Solal AC, Joubert D, Legraverend C. Visualization of S100B-positive neurons and glia in the central nervous system of EGFP transgenic mice. J Comp Neurol. 2003 Mar 17;457(4):404-19. | 12561079 | | Yokoo H, Tanaka Y, Nobusawa S, Nakazato Y, Ohgaki H. Immunohistochemical and ultrastructural characterization of brain tumors in S100beta-v-erbB transgenic rats. Neuropathology. 2008 May 21; [Epub ahead of print] | 18498286 | | Itakura E, Odaira K, Yokoyama K, Osuna M, Hara T, Inoue K. Generation of transgenic rats expressing green fluorescent protein in S-100beta-producing pituitary folliculo-stellate cells and brain astrocytes. Endocrinology. 2007 Apr;148(4):1518-23. Epub 2007 Jan 18. | 17234709 | | Maeda T, Usui H, Araki K, Kuwano R, Takahashi Y, Suzuki Y. Structure and expression of rat S-100 beta subunit gene. Brain Res Mol Brain Res. 1991 Jun;10(3):193-202. | 1653388 | | Pena LA, Brecher CW, Marshak DR. beta-Amyloid regulates gene expression of glial trophic substance S100 beta in C6 glioma and primary astrocyte cultures. Brain Res Mol Brain Res. 1995 Dec 1;34(1):118-26. | 8750867 | | Hinkle DA, Harney JP, Cai A, Hilt DC, Yarowsky PJ, Wise PM. Basic fibroblast growth factor-2 and interleukin-1 beta regulate S100 beta expression in cultured astrocytes. Neuroscience. 1998 Jan;82(1):33-41. | 9483501 | | Ohgaki H, Kita D, Favereaux A, Huang H, Homma T, Dessen P, Weiss WA, Kleihues P, Heppner FL. Brain tumors in S100beta-v-erbB transgenic rats. J Neuropathol Exp Neurol. 2006 Dec;65(12):1111-7. | 17146284 | | Weiss WA, Burns MJ, Hackett C, Aldape K, Hill JR, Kuriyama H, Kuriyama N, Milshteyn N, Roberts T, Wendland MF, DePinho R, Israel MA. Genetic determinants of malignancy in a mouse model for oligodendroglioma. Cancer Res. 2003 Apr 1;63(7):1589-95. | 12670909 | | Darlot F, Artuso A, Lautredou-Audouy N, Casellas D. Topology of Schwann Cells and Sympathetic Innervation along Preglomerular vessels. A Confocal Microscopic Study in Protein S100B / EGFP Transgenic Mice. Am J Physiol Renal Physiol. 2008 Aug 13; [Epub ahead of print] | 18701627 | | Tomita K, Hata Y, Kubo T, Fujiwara T, Yano K, Hosokawa K. Effects of the in vivo predegenerated nerve graft on early Schwann cell migration: Quantitative analysis using S100-GFP mice. Neurosci Lett. 2009 Jun 8; [Epub ahead of print] | 19500656 |
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* The Ple185 MiniPromoter (Patent Pending) is proprietary - any commercial
use of the MiniPromoter will require a direct license from The University of British
Columbia. Please direct all licensing inquiries to Elizabeth M. Simpson, PhD,
simpson@cmmt.ubc.ca Office:
604-875-3830
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