Ple118-EGFP (Negative)
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Other constructs for this MiniPromoter: NONE |
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Other constructs for this Gene: Ple119-lacZ (Positive) |
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Ple118-EGFP Description:
The Ple118 promoter construct is derived from the promoter region of the 5-hydroxytryptamine 1A receptor (HTR1A) gene. The Pleiades Promoter Project constructed a 3102 bp MiniPromoter from an upstream putative regulatory element and a subsection of the HTR1A promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1387 with Ple118 driving EGFP. The Ple118-EGFP (pEMS1387) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS1675, mEMS1676, mEMS1808, and mEMS1809) were PCR validated, and chimeras were generated using mEMS2216.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple118-EGFP Expression Pattern:
No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).
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Ple118 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Ansorge M, Tanneberger C, Davies B, Theuring F, Kusserow H. Analysis of the murine 5-HT receptor gene promoter in vitro and in vivo. Eur J Neurosci. 2004 Jul;20(2):363-74. | 15233746 | | Parks CL, Shenk T. The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1. J Biol Chem. 1996 Feb 23;271(8):4417-30. | 8626793 | | Storring JM, Charest A, Cheng P, Albert PR. TATA-driven transcriptional initiation and regulation of the rat serotonin 5-HT1A receptor gene. J Neurochem. 1999 Jun;72(6):2238-47. | 10349831 | | 0 | | Meijer OC, Williamson A, Dallman MF, Pearce D. Transcriptional repression of the 5-HT1A receptor promoter by corticosterone via mineralocorticoid receptors depends on the cellular context. J Neuroendocrinol. 2000 Mar;12(3):245-54. | 10718920 | | Wissink S, Meijer O, Pearce D, van Der Burg B, van Der Saag PT. Regulation of the rat serotonin-1A receptor gene by corticosteroids. J Biol Chem. 2000 Jan 14;275(2):1321-6. | 10625680 | | Ou XM, Storring JM, Kushwaha N, Albert PR. Heterodimerization of mineralocorticoid and glucocorticoid receptors at a novel negative response element of the 5-HT1A receptor gene. J Biol Chem. 2001 Apr 27;276(17):14299-307. Epub 2001 Feb 2. | 11278286 | | Wissink S, van der Burg B, Katzenellenbogen BS, van der Saag PT. Synergistic activation of the serotonin-1A receptor by nuclear factor-kappa B and estrogen. Mol Endocrinol. 2001 Apr;15(4):543-52. | 11266506 | | Bonnin A, Peng W, Hewlett W, Levitt P. Expression mapping of 5-HT1 serotonin receptor subtypes during fetal and early postnatal mouse forebrain development. Neuroscience. 2006 Aug 25;141(2):781-94. Epub 2006 Jul 7. | 16824687 | | Pompeiano M, Palacios JM, Mengod G. Distribution and cellular localization of mRNA coding for 5-HT1A receptor in the rat brain: correlation with receptor binding. J Neurosci. 1992 Feb;12(2):440-53. | 1531498 | | Miquel MC, Kia HK, Boni C, Doucet E, Daval G, Matthiessen L, Hamon M, Verge D. Postnatal development and localization of 5-HT1A receptor mRNA in rat forebrain and cerebellum. Brain Res Dev Brain Res. 1994 Jul 15;80(1-2):149-57. | 7955340 | | Chalmers DT, Watson SJ. Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain--a combined in situ hybridisation/in vitro receptor autoradiographic study. Brain Res. 1991 Oct 4;561(1):51-60. | 1797349 | | Chalmers DT, Kwak SP, Mansour A, Akil H, Watson SJ. Corticosteroids regulate brain hippocampal 5-HT1A receptor mRNA expression. J Neurosci. 1993 Mar;13(3):914-23. | 8441016 | | Zhong P, Ciaranello RD. Transcriptional regulation of hippocampal 5-HT1a receptors by corticosteroid hormones. Brain Res Mol Brain Res. 1995 Mar;29(1):23-34. | 7769998 | | Czesak M, Lemonde S, Peterson EA, Rogaeva A, Albert PR. Cell-specific repressor or enhancer activities of Deaf-1 at a serotonin 1A receptor gene polymorphism. J Neurosci. 2006 Feb 8;26(6):1864-71. | 16467535 | | Association of the C(-1019)G 5-HT1A functional promoter polymorphism with antidepressant response. Comment in: Int J Neuropsychopharmacol. 2004 Dec;7(4):381-5. Int J Neuropsychopharmacol. 2004 Dec;7(4):501-6. Epub 2004 Sep 27. | 15447813 | | Rogaeva A, Albert PR. The mental retardation gene CC2D1A/Freud-1 encodes a long isoform that binds conserved DNA elements to repress gene transcription. Eur J Neurosci. 2007 Aug;26(4):965-74. | 17714190 | | Albert PR, Zhou QY, Van Tol HH, Bunzow JR, Civelli O. Cloning, functional expression, and mRNA tissue distribution of the rat 5-hydroxytryptamine1A receptor gene. J Biol Chem. 1990 Apr 5;265(10):5825-32. | 2156831 | | Ou XM, Jafar-Nejad H, Storring JM, Meng JH, Lemonde S, Albert PR. Novel dual repressor elements for neuronal cell-specific transcription of the rat 5-HT1A receptor gene. J Biol Chem. 2000 Mar 17;275(11):8161-8. | 10713139 | | Lemonde S, Rogaeva A, Albert PR. Cell type-dependent recruitment of trichostatin A-sensitive repression of the human 5-HT1A receptor gene. J Neurochem. 2004 Feb;88(4):857-68. | 14756806 |
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