The Pleiades Promoter Project

All MiniPromoters

Ple155-EGFP (Negative)

Other constructs for this MiniPromoter: Ple155-lacZ (Positive)

Other constructs for this Gene: Ple154-Cre/EGFP (Negative), Ple157-EGFP (Negative)

Ple155-EGFP Description:

The Ple155 promoter construct is derived from the promoter region of the Purkinje cell protein 2 (L7/PCP2) gene. The Pleiades Promoter Project created a 1648 bp MiniPromoter derived from a combination of the promoters upstream and downstream of the first exon and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1424 with Ple155 driving EGFP. The Ple155-EGFP (pEMS1424) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204.03A. Four ESC lines (mEMS1725, mEMS1738, mEMS1740, and mEMS1743) were PCR validated. Chimeras were generated using mEMS1738.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple155-EGFP Expression Pattern:

No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).

Ple155 MiniPromoter Resources:

Ple155-EGFP
	plasmid pEMS1424 (in backbone pEMS1306)
	ESCs mEMS1725, mEMS1738, mEMS1740, mEMS1743
	mice generated using ESC mEMS1738
Resource Files
	Ple155 pEMS1424 Sequence	Jones lab
	Ple155 pEMS1424 MiniPromoter Design	Wasserman lab
	Ple155 pEMS1424 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple155 pEMS1424 Vector Map	Holt lab
	Ple155 pEMS1424 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID
Hashimoto M, Aruga J, Hosoya Y, Kanegae Y, Saito I, Mikoshiba K. A neural cell-type-specific expression system using recombinant adenovirus vectors. Hum Gene Ther. 1996 Jan 20;7(2):149-58.	8788166
Matsui T. Transcriptional regulation of a Purkinje cell-specific gene through a functional interaction between ROR alpha and RAR. Genes Cells. 1997 Apr;2(4):263-72.	9224660
Anderson GW, Hagen SG, Larson RJ, Strait KA, Schwartz HL, Mariash CN, Oppenheimer JH. Purkinje cell protein-2 cis-elements mediate repression of T3-dependent transcriptional activation. Mol Cell Endocrinol. 1997 Jul 4;131(1):79-87.	9256366
Anderson GW, Larson RJ, Oas DR, Sandhofer CR, Schwartz HL, Mariash CN, Oppenheimer JH. Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) modulates expression of the Purkinje cell protein-2 gene. A potential role for COUP-TF in repressing premature thyroid hormone action in the developing brain. J Biol Chem. 1998 Jun 26;273(26):16391-9.	9632703
Zhang XM, Ng AH, Tanner JA, Wu WT, Copeland NG, Jenkins NA, Huang JD. Highly restricted expression of Cre recombinase in cerebellar Purkinje cells. Genesis. 2004 Sep;40(1):45-51.	15354293
Zhang X, Baader SL, Bian F, Muller W, Oberdick J. High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Histochem Cell Biol. 2001 Jun;115(6):455-64.	11455445
Sanlioglu S, Zhang X, Baader SL, Oberdick J. Regulation of a Purkinje cell-specific promoter by homeodomain proteins: repression by engrailed-2 vs. synergistic activation by Hoxa5 and Hoxb7. J Neurobiol. 1998 Sep 15;36(4):559-71.	9740027
Oberdick J, Smeyne RJ, Mann JR, Zackson S, Morgan JI. A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons. Science. 1990 Apr 13;248(4952):223-6.	2109351
Vandaele S, Nordquist DT, Feddersen RM, Tretjakoff I, Peterson AC, Orr HT. Purkinje cell protein-2 regulatory regions and transgene expression in cerebellar compartments. Genes Dev. 1991 Jul;5(7):1136-48.	2065970
Barski JJ, Dethleffsen K, Meyer M. Cre recombinase expression in cerebellar Purkinje cells. Genesis. 2000 Nov-Dec;28(3-4):93-8.	11105049
Anderson L, Rossi D, Linehan J, Brandner S, Weissmann C. Transgene-driven expression of the Doppel protein in Purkinje cells causes Purkinje cell degeneration and motor impairment. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3644-9. Epub 2004 Mar 8.	15007176
Ichise T, Kano M, Hashimoto K, Yanagihara D, Nakao K, Shigemoto R, Katsuki M, Aiba A. mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination. Science. 2000 Jun 9;288(5472):1832-5.	10846166
Local control of granule cell generation by cerebellar Purkinje cells. Erratum in: Mol Cell Neurosci. 2006 May-Jun;32(1-2):215. S-Crisman, S [corrected to Sanlioglu, S]. Mol Cell Neurosci. 1995 Jun;6(3):230-51.	7496629
Smeyne RJ, Oberdick J, Schilling K, Berrebi AS, Mugnaini E, Morgan JI. Dynamic organization of developing Purkinje cells revealed by transgene expression. Science. 1991 Nov 1;254(5032):719-21.	1948052
Oberdick J, Schilling K, Smeyne RJ, Corbin JG, Bocchiaro C, Morgan JI. Control of segment-like patterns of gene expression in the mouse cerebellum. Neuron. 1993 Jun;10(6):1007-18.	8318226