Ple3-lacZ (Positive)
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Other constructs for this MiniPromoter: Ple3-EGFP (Negative) |
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Other constructs for this Gene: Ple2-EGFP (Negative), Ple4-EGFP (Negative) |
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Ple3-lacZ Description:
The Ple3 promoter construct is derived from the promoter region of the Adenosine A2a receptor (ADORA2A) gene. The Pleiades Promoter Project created a 1801 bp MiniPromoter derived from two upstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1473 with Ple3 driving lacZ. The Ple3-lacZ (pEMS1473) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS3986, mEMS3712, mEMS4048, and mEMS4056. Chimeras were generated using mEMS3986.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple3-lacZ Expression Pattern:
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Ple3 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Lee YC, Lai HL, Sun CN, Chien CL, Chern Y. Identification of nuclear factor 1 (NF1) as a transcriptional modulator of rat A(2A) adenosine receptor. Brain Res Mol Brain Res. 2003 Mar 17;111(1-2):61-73. | 12654506 | | Lee YC, Chien CL, Sun CN, Huang CL, Huang NK, Chiang MC, Lai HL, Lin YS, Chou SY, Wang CK, Tai MH, Liao WL, Lin TN, Liu FC, Chern Y. Characterization of the rat A2A adenosine receptor gene: a 4.8-kb promoter-proximal DNA fragment confers selective expression in the central nervous system. Eur J Neurosci. 2003 Oct;18(7):1786-96. | 14622213 | | Yu L, Frith MC, Suzuki Y, Peterfreund RA, Gearan T, Sugano S, Schwarzschild MA, Weng Z, Fink JS, Chen JF. Characterization of genomic organization of the adenosine A2A receptor gene by molecular and bioinformatics analyses. Brain Res. 2004 Mar 12;1000(1-2):156-73. | 15053963 | | Chiang MC, Lee YC, Huang CL, Chern Y. cAMP-response element-binding protein contributes to suppression of the A2A adenosine receptor promoter by mutant Huntingtin with expanded polyglutamine residues. J Biol Chem. 2005 Apr 8;280(14):14331-40. Epub 2005 Feb 2. | 15689617 | | Yu L, Haverty PM, Mariani J, Wang Y, Shen HY, Schwarzschild MA, Weng Z, Chen JF. Genetic and pharmacological inactivation of adenosine A2A receptor reveals an Egr-2-mediated transcriptional regulatory network in the mouse striatum. Physiol Genomics. 2005 Sep 21;23(1):89-102. Epub 2005 Jul 26. | 16046619 | | Lee YC, Chang CW, Su CW, Lin TN, Sun SH, Lai HL, Chern Y. The 5' untranslated regions of the rat A2A adenosine receptor gene function as negative translational regulators. J Neurochem. 1999 Nov;73(5):1790-8. | 10537036 | | Morello S, Ito K, Yamamura S, Lee KY, Jazrawi E, Desouza P, Barnes P, Cicala C, Adcock IM. IL-1 beta and TNF-alpha regulation of the adenosine receptor (A2A) expression: differential requirement for NF-kappa B binding to the proximal promoter. J Immunol. 2006 Nov 15;177(10):7173-83. | 17082635 | | Borgland SL, Castanon M, Spevak W, Parkinson FE. Effects of propentofylline on adenosine receptor activity in Chinese hamster ovary cell lines transfected with human A1, A2A, or A2B receptors and a luciferase reporter gene. Can J Physiol Pharmacol. 1998 Dec;76(12):1132-8. | 10326835 | | Chu YY, Tu KH, Lee YC, Kuo ZJ, Lai HL, Chern Y. Characterization of the rat A2a adenosine receptor gene. DNA Cell Biol. 1996 Apr;15(4):329-37. | 8639269 | | Kreth S, Ledderose C, Kaufmann I, Groeger G, Thiel M. Differential expression of 5'-UTR splice variants of the adenosine A2A receptor gene in human granulocytes: identification, characterization, and functional impact on activation. FASEB J. 2008 Jun 9; [Epub ahead of print] | 18541693 | | Popat RA, Van Den Eeden SK, Tanner CM, Kamel F, Umbach DM, Marder K, Mayeux R, Ritz B, Ross GW, Petrovitch H, Topol B, McGuire V, Costello S, Manthripragada AD, Southwick A, Myers RM, Nelson LM. Coffee, ADORA2A, and CYP1A2: the caffeine connection in Parkinson's disease. Eur J Neurol. 2011 Jan 31; [Epub ahead of print] | 21281405 |
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