This Ple17 promoter construct is derived from the promoter region of the gene C8ORF46. The Pleiades Promoter Project created a 2603 bp MiniPromoter derived from three downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1487 with Ple17 driving lacZ. The Ple17-lacZ (pEMS1487) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4619, mEMS4612, mEMS4620, and mEMS4622. Chimeras were generated using mEMS4619.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).