Ple17-lacZ (Positive)

Ple17-lacZ Description:

This Ple17 promoter construct is derived from the promoter region of the gene C8ORF46. The Pleiades Promoter Project created a 2603 bp MiniPromoter derived from three downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1487 with Ple17 driving lacZ. The Ple17-lacZ (pEMS1487) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4619, mEMS4612, mEMS4620, and mEMS4622. Chimeras were generated using mEMS4619.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple17 MiniPromoter Resources:

Ple17-lacZ
	plasmid pEMS1487 (in backbone pEMS1313)
	ESCs mEMS4619, mEMS4612, mEMS4620, mEMS4622
	mice generated using ESC mEMS4619
Resource Files
	Ple17 pEMS1487 Sequence	Jones lab
	Ple17 pEMS1487 MiniPromoter Design	Wasserman lab
	Ple17 pEMS1487 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple17 pEMS1487 Vector Map	Holt lab
	Ple17 pEMS1487 Genotyping Assay  (Requires VectorNTI Sofware)	Holt lab