The Pleiades Promoter Project

All MiniPromoters

CAG-lacZ (Positive)

Other Constructs for CAG: CAG-EGFP (pEMS1157; Positive), CAG-EGFP (pEMS1277; Positive)


7.4 weeks, N2, Male (entire series)	11.3 weeks, N2, Male (entire series)

CAG-lacZ Description:

The Pleiades Promoter Project obtained the CAG promoter (a composite of the Chicken beta-actin promoter and 5' UTR, along with a Human CMV enhancer) and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 to make plasmid pEMS1488 with CAG driving constitutive expression of lacZ. The CAG-lacZ (pEMS1488) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. One ESC line (mEMS3960) was PCR validated. Chimeras were generated using mEMS3960.
This is a positive control.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have also been generated.

CAG Promoter Resources:

CAG-lacZ
	plasmid pEMS1488 (in backbone pEMS1313)
	ESCs mEMS3960
	mice generated using ESC mEMS3960
Resource Files
	CAG Sequence	Jones lab
	CAG MiniPromoter Design	Wasserman lab
	CAG pEMS1488 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	CAG pEMS1488 Vector Map	Holt lab
	CAG pEMS1488 Genotyping Assay	Simpson lab

Publications:

Reference	PubMed ID
Miyazaki J, Takaki S, Araki K, Tashiro F, Tominaga A, Takatsu K, Yamamura K. Expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5. Gene. 1989 Jul 15;79(2):269-77.	2551778
Niwa H, Yamamura K, Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991 Dec 15;108(2):193-9.	1660837

CAG-lacZ (Positive)

CAG-lacZ Description:

CAG-lacZ Expression Pattern:

CAG Promoter Resources:

Publications: