This Ple24 promoter construct is derived from the promoter region of the Gastrin / Cholecystokinin type B receptor (CCK-B receptor) (CCKBR) gene. The Pleiades Promoter Project created a 3808 bp MiniPromoter derived from two downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1495 with Ple24 driving lacZ. The Ple24-lacZ (pEMS1495) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4699, mEMS4704, mEMS4706, and mEMS4679. Chimeras were generated using mEMS4699.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).