Ple24-lacZ (Positive)

Ple24-lacZ Description:

This Ple24 promoter construct is derived from the promoter region of the Gastrin / Cholecystokinin type B receptor (CCK-B receptor) (CCKBR) gene. The Pleiades Promoter Project created a 3808 bp MiniPromoter derived from two downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1495 with Ple24 driving lacZ. The Ple24-lacZ (pEMS1495) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4699, mEMS4704, mEMS4706, and mEMS4679. Chimeras were generated using mEMS4699.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple24 MiniPromoter Resources:

Ple24-lacZ
	plasmid pEMS1495 (in backbone pEMS1313)
	ESCs mEMS4699, mEMS4704, mEMS4706, mEMS4679
	mice generated using ESC mEMS4699
Resource Files
	Ple24 pEMS1495 Sequence	Jones lab
	Ple24 pEMS1495 MiniPromoter Design	Wasserman lab
	Ple24 pEMS1495 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple24 pEMS1495 Vector Map	Holt lab
	Ple24 pEMS1495 Genotyping Assay  (Requires VectorNTI Sofware)	Holt lab