Ple25-lacZ (Positive)

Ple25-lacZ Description:

The Ple25 promoter construct is derived from the promoter region of the Gastrin / Cholecystokinin type B receptor (CCK-B receptor) (CCKBR) gene. The Pleiades Promoter Project created a 3704 bp MiniPromoter derived from two downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1496 with Ple25 driving lacZ. The Ple25-lacZ (pEMS1496) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4627, mEMS4628, mEMS4632, and mEMS4633. Chimeras were generated using mEMS4627.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple25 MiniPromoter Resources:

Ple25-lacZ
	plasmid pEMS1496 (in backbone pEMS1313)
	ESCs mEMS4627, mEMS4628, mEMS4632, mEMS4633
	mice generated using ESC mEMS4627
Resource Files
	Ple25 pEMS1496 Sequence	Jones lab
	Ple25 pEMS1496 MiniPromoter Design	Wasserman lab
	Ple25 pEMS1496 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple25 pEMS1496 Vector Map	Holt lab
	Ple25 pEMS1496 Genotyping Assay  (Requires VectorNTI Sofware)	Holt lab