Ple35-lacZ (Positive)

Ple35-lacZ Description:

The Ple35 promoter construct is derived from the promoter region of the Claudin 5 (CLDN5) gene. The Pleiades Promoter Project created a 2826 bp MiniPromoter derived from one upstream putative regulatory subregion along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1506 with Ple35 driving lacZ. The Ple35-lacZ (pEMS1506) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4234, mEMS4225, mEMS4241, and mEMS4231. Chimeras were generated using mEMS4234.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple35 MiniPromoter Resources:

Ple35-lacZ
	plasmid pEMS1506 (in backbone pEMS1313)
	ESCs mEMS4234, mEMS4225, mEMS4241, mEMS4231
	chimeras generated using ESC mEMS4234
Resource Files
	Ple35 pEMS1506 Sequence	Jones lab
	Ple35 pEMS1506 MiniPromoter Design	Wasserman lab

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