The Pleiades Promoter Project

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All MiniPromoters

Ple53-lacZ (Positive)

Other constructs for this MiniPromoter: Ple53-EGFP (Positive)

Other constructs for this Gene: Ple54-EGFP (Positive), Ple55-EGFP (Positive), Ple55-lacZ (Positive)

8.1 weeks, N2, Male
(entire series)

Ple53-lacZ Description:

The Ple53 promoter construct is derived from the promoter region of the Doublecortin (DCX) gene. The Pleiades Promoter Project created a 3520 bp MiniPromoter derived from a subsection of the DCX promoter. This construct was introduced into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1524 with Ple53 driving lacZ. The Ple53-lacZ (pEMS1524) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1202. Four ESC lines (mEMS3731, mEMS3727, mEMS3730, and mEMS3737) were PCR validated. Chimeras were generated using mEMS3731.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple53 MiniPromoter Resources:

Ple53-lacZ
	plasmid pEMS1524 (in backbone pEMS1313)
	ESCs mEMS3731, mEMS3727, mEMS3730, mEMS3737
	mice generated using ESC mEMS3731
Resource Files
	Ple53 pEMS1524 Sequence	Jones lab
	Ple53 pEMS1524 MiniPromoter Design	Wasserman lab
	Ple53 pEMS1524 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple53 pEMS1524 Vector Map	Holt lab
	Ple53 pEMS1524 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID
Karl C, Couillard-Despres S, Prang P, Munding M, Kilb W, Brigadski T, Plotz S, Mages W, Luhmann H, Winkler J, Bogdahn U, Aigner L. Neuronal precursor-specific activity of a human doublecortin regulatory sequence. J Neurochem. 2005 Jan;92(2):264-82.	15663475
Couillard-Despres S, Winner B, Karl C, Lindemann G, Schmid P, Aigner R, Laemke J, Bogdahn U, Winkler J, Bischofberger J, Aigner L. Targeted transgene expression in neuronal precursors: watching young neurons in the old brain. Eur J Neurosci. 2006 Sep;24(6):1535-45.	17004917
Wang X, Mao X, Xie L, Greenberg DA, Jin K. Involvement of Notch1 signaling in neurogenesis in the subventricular zone of normal and ischemic rat brain in vivo. J Cereb Blood Flow Metab. 2009 Jun 17; [Epub ahead of print]	19536070
Piens M, Muller M, Bodson M, Baudouin G, Plumier JC. A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons. BMC Neurosci. 2010 May 28;11(1):64 [Epub ahead of print]	20509865

Ple53-lacZ (Positive)

Ple53-lacZ Description:

Ple53-lacZ Expression Pattern:

Ple53 MiniPromoter Resources:

Publications: