The Pleiades Promoter Project

All MiniPromoters

Ple55-lacZ (Positive)

Other constructs for this MiniPromoter: Ple55-EGFP (Positive)

Other constructs for this Gene: Ple53-EGFP (Positive), Ple53-lacZ (Positive), Ple54-EGFP (Positive)


8 weeks, N2, Male (entire series)	13 weeks, N1, Female (entire series)

Ple55-lacZ Description:

The Ple55 promoter construct is derived from the promoter region of the Doublecortin (DCX) gene. The Pleiades Promoter Project created a 3877 bp MiniPromoter derived from one downstream putative regulatory subregion along with a section of the promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1526 with Ple55 driving lacZ. The Ple55-lacZ (pEMS1526) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS3879, mEMS3870, mEMS3860, and mEMS3884. Chimeras were generated using mEMS3879.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple55 MiniPromoter Resources:

Ple55-lacZ
	plasmid pEMS1526 (in backbone pEMS1313)
	ESCs mEMS3879, mEMS3870, mEMS3860, mEMS3884
	mice generated using ESC mEMS3879
Resource Files
	Ple55 pEMS1526 Sequence	Jones lab
	Ple55 pEMS1526 MiniPromoter Design	Wasserman lab
	Ple55 pEMS1526 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple55 pEMS1526 Vector Map	Holt lab
	Ple55 pEMS1526 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID
Karl C, Couillard-Despres S, Prang P, Munding M, Kilb W, Brigadski T, Plotz S, Mages W, Luhmann H, Winkler J, Bogdahn U, Aigner L. Neuronal precursor-specific activity of a human doublecortin regulatory sequence. J Neurochem. 2005 Jan;92(2):264-82.	15663475
Couillard-Despres S, Winner B, Karl C, Lindemann G, Schmid P, Aigner R, Laemke J, Bogdahn U, Winkler J, Bischofberger J, Aigner L. Targeted transgene expression in neuronal precursors: watching young neurons in the old brain. Eur J Neurosci. 2006 Sep;24(6):1535-45.	17004917
Wang X, Mao X, Xie L, Greenberg DA, Jin K. Involvement of Notch1 signaling in neurogenesis in the subventricular zone of normal and ischemic rat brain in vivo. J Cereb Blood Flow Metab. 2009 Jun 17; [Epub ahead of print]	19536070
Piens M, Muller M, Bodson M, Baudouin G, Plumier JC. A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons. BMC Neurosci. 2010 May 28;11(1):64 [Epub ahead of print]	20509865

Ple55-lacZ (Positive)

Ple55-lacZ Description:

Ple55-lacZ Expression Pattern:

Ple55 MiniPromoter Resources:

Publications: