The Pleiades Promoter Project

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All MiniPromoters

Ple97-lacZ (Positive)

Other constructs for this MiniPromoter: Ple97-EGFP (Negative)

Other constructs for this Gene: Ple98-EGFP (Negative)

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Ple97-lacZ Description:

This Ple97 promoter construct is derived from the promoter region of the Glutathione peroxidase 3 (GPX3) gene. The Pleiades Promoter Project created a 3787 bp MiniPromoter derived from two upstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1568 with Ple97 driving lacZ. The Ple97-lacZ (pEMS1568) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4232, mEMS4222, mEMS4228, and mEMS4236. Chimeras were generated using mEMS4232.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple97 MiniPromoter Resources:

Ple97-lacZ
	plasmid pEMS1568 (in backbone pEMS1313)
	ESCs mEMS4222, mEMS4228, mEMS4232, mEMS4236
	mice generated using ESC mEMS4232
Resource Files
	Ple97 pEMS1568 Sequence	Jones lab
	Ple97 pEMS1568 MiniPromoter Design	Wasserman lab
	Ple97 pEMS1568 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple97 pEMS1568 Vector Map	Holt lab
	Ple97 pEMS1568 Genotyping Assay (Requires VectorNTI Sofware)	Holt lab

Publications:

Reference	PubMed ID
Bierl C, Voetsch B, Jin RC, Handy DE, Loscalzo J. Determinants of human plasma glutathione peroxidase (GPx-3) expression. J Biol Chem. 2004 Jun 25;279(26):26839-45. Epub 2004 Apr 19.	15096516
Promoter polymorphisms in the plasma glutathione peroxidase (GPx-3) gene: a novel risk factor for arterial ischemic stroke among young adults and children. Comment in: Stroke. 2007 Jan;38(1):6. Stroke. 2007 Jun;38(6):e23; author reply e24. Stroke. 2007 Jan;38(1):41-9. Epub 2006 Nov 22.	17122425
Comhair SA, Bhathena PR, Farver C, Thunnissen FB, Erzurum SC. Extracellular glutathione peroxidase induction in asthmatic lungs: evidence for redox regulation of expression in human airway epithelial cells. FASEB J. 2001 Jan;15(1):70-78.	11149894
Voetsch B, Jin RC, Bierl C, Deus-Silva L, Camargo EC, Annichino-Bizacchi JM, Handy DE, Loscalzo J. Role of promoter polymorphisms in the plasma glutathione peroxidase (GPx-3) gene as a risk factor for cerebral venous thrombosis. Stroke. 2008 Feb;39(2):303-7. Epub 2007 Dec 20.	18096833
Jee CD, Kim MA, Jung EJ, Kim J, Kim WH. Identification of genes epigenetically silenced by CpG methylation in human gastric carcinoma. Eur J Cancer. 2009 Feb 3; [Epub ahead of print]	19195878

Ple97-lacZ (Positive)

Ple97-lacZ Description:

Ple97-lacZ Expression Pattern:

Ple97 MiniPromoter Resources:

Publications: