Ple123-lacZ (Positive)

Ple123-lacZ Description:

The Ple123 promoter construct is derived from the promoter region of the Isoprenylcysteine carboxyl methyltransferase (ICMT) gene. The Pleiades Promoter Project created a 2342 bp MiniPromoter derived from three downstream putative regulatory subregion along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1594 with Ple123 driving lacZ. The Ple123-lacZ (pEMS1594) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4556, mEMS4548, mEMS4549, and mEMS4567. Chimeras were generated using mEMS4556.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple123 MiniPromoter Resources:

Ple123-lacZ
	plasmid pEMS1594 (in backbone pEMS1313)
	ESCs mEMS4556, mEMS4548, mEMS4549, mEMS4567
	mice generated using ESC mEMS4556
Resource Files
	Ple123 pEMS1594 Sequence	Jones lab
	Ple123 pEMS1594 MiniPromoter Design	Wasserman lab
	Ple123 pEMS1594 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple123 pEMS1594 Vector Map	Holt lab
	Ple123 pEMS1594 Genotyping Assay  (Requires VectorNTI Sofware)	Holt lab

Publications: