Ple160-lacZ (Positive)
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Other constructs for this MiniPromoter: Ple160-EGFP/cre (Negative), Ple160-EGFP (Negative) |
Other constructs for this Gene: Ple161-EGFP/cre (Negative), Ple162-EGFP/cre (Positive),
Ple161-EGFP (Negative), Ple162-EGFP (Negative) |
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Ple160-lacZ Description:
This Ple160 promoter construct is derived from the promoter region of the Pituitary homeobox 3 (PITX3) gene. The Pleiades Promoter Project created a 2534 bp MiniPromoter derived from a segment of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1631 with Ple160 driving lacZ. The Ple160-lacZ (pEMS1631) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4135, mEMS4160, mEMS4140, and mEMS4169. Chimeras were generated using mEMS4135.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple160-lacZ Expression Pattern:
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Ple160 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Semina EV, Murray JC, Reiter R, Hrstka RF, Graw J. Deletion in the promoter region and altered expression of Pitx3 homeobox gene in aphakia mice. Hum Mol Genet. 2000 Jul 1;9(11):1575-85. | 10861284 | | Smidt MP, Smits SM, Bouwmeester H, Hamers FP, van der Linden AJ, Hellemons AJ, Graw J, Burbach JP. Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3. Development. 2004 Mar;131(5):1145-55. | 14973278 | | Smidt MP, van Schaick HS, Lanctot C, Tremblay JJ, Cox JJ, van der Kleij AA, Wolterink G, Drouin J, Burbach JP. A homeodomain gene Ptx3 has highly restricted brain expression in mesencephalic dopaminergic neurons. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13305-10. | 9371841 | | Rieger DK, Reichenberger E, McLean W, Sidow A, Olsen BR. A double-deletion mutation in the Pitx3 gene causes arrested lens development in aphakia mice. Genomics. 2001 Feb 15;72(1):61-72. | 11247667 | | Maxwell SL, Ho HY, Kuehner E, Zhao S, Li M. Pitx3 regulates tyrosine hydroxylase expression in the substantia nigra and identifies a subgroup of mesencephalic dopaminergic progenitor neurons during mouse development. Dev Biol. 2005 Jun 15;282(2):467-79. | 15950611 | | Coulon V, L'Honore A, Ouimette JF, Dumontier E, van den Munckhof P, Drouin J. A muscle-specific promoter directs Pitx3 gene expression in skeletal muscle cells. J Biol Chem. 2007 Nov 9;282(45):33192-200. Epub 2007 Sep 11. | 17848564 | | Zhao S, Maxwell S, Jimenez-Beristain A, Vives J, Kuehner E, Zhao J, O'Brien C, de Felipe C, Semina E, Li M. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons. Eur J Neurosci. 2004 Mar;19(5):1133-40. | 15016072 | | Castillo-Carranza DL, Rodriguez-Rocha H, Montes-de-Oca-Luna R, Sepulveda-Saavedra J, Martinez HR, Lopez-Vidal Y, Saucedo-Cardenas O. Pitx3 promoter directs Cre-recombinase specifically in a human neuroblastoma cell line. Mol Cell Biochem. 2008 Feb;309(1-2):223-7. Epub 2007 Nov 30. | 18049867 | | Hedlund EM, Pruszak J, Lardaro T, Ludwig W, Vinuela A, Kim KS, Isacson O. Embryonic Stem (ES) Cell-derived Pitx3-eGFP Midbrain Dopamine Neurons Survive Enrichment by FACS and Function in an Animal Model of Parkinson's Disease. Stem Cells. 2008 Apr 3; [Epub ahead of print] | 18388307 | | 0 | | Qiu HY, Guo C, Cheng XW, Huang Y, Xiong ZQ, Ding YQ. Pitx3-CreER mice showing restricted Cre expression in developing ocular lens and skeletal muscles. Genesis. 2008 Jun;46(6):324-8. | 18543300 | | Doni A, Mantovani G, Porta C, Tuckermann J, Reichardt HM, Kleiman A, Sironi M, Rubino L, Pasqualini F, Nebuloni M, Signorini S, Peri G, Sica A, Beck-Peccoz P, Bottazzi B, Mantovani A. Cell-specific regulation of PTX3 by glucocorticoid hormones in hematopoietic and non-hematopoietic cells. J Biol Chem. 2008 Aug 14; [Epub ahead of print] | 18703503 | | Jacobs FM, Van der Linden AJ, Wang Y, von Oerthel L, Sul HS, Burbach JP, Smidt MP. Identification of Dlk1, Ptpru and Klhl1 as novel Nurr1 target genes in meso-diencephalic dopamine neurons. Development. 2009 Jun 10; [Epub ahead of print] | 19515692 | | Konstantoulas CJ, Parmar M, Li M. FoxP1 promotes midbrain identity in embryonic stem cell derived dopamine neurons by regulating Pitx3. J Neurochem. 2010 Feb 19; [Epub ahead of print] | 20175877 | | Yu LH, Lin ZF, Liu Y, Hu FY, He XH, Liu ZL, Xu YM. The transcription factor Pitx3 is a risk modifier for Parkinson's disease in a Chinese Han population. Eur J Neurol. 2010 Dec 7; [Epub ahead of print] | 21138504 |
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